Hormone-injected leaf cutting, a new efficient in vivo multiplication protocol for two succulent plants

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1 Faculty of Art and Communication, Kunming University of Science and Technology, Kunming, Yunnan, China
2 Faculty of Architecture and City Planning, Kunming University of Science and Technology, Kunming, Yunnan, China
DOI
10.7287/peerj.preprints.2656v2
Published
Accepted
Subject Areas
Agricultural Science, Plant Science
Keywords
bud multiplication, direct organogenesis, Sedum morganianum, Pachyveria pachytoides
Copyright
© 2017 Xu et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Xu X, Zheng W. 2017. Hormone-injected leaf cutting, a new efficient in vivo multiplication protocol for two succulent plants. PeerJ Preprints 5:e2656v2

Abstract

This study aimed to establish a simple and efficient in vivo multiplication protocol by leaf cutting to satisfy the supply of young succulent ornamentals Pachyveria pachytoides and Sedum morganianum. The regenerability of leaves injected with 6-benzylaminopurine (BAP) and α-naphthalene acetic acid (NAA) in vivo were tested with common leaf cutting as control. Results showed a 100% shoot induction frequency using hormone-injeceted methods for the two species. The number of shoots per leaf of 4.0 or 6.0 mg l −1 BAP and 0.1 mg l −1 NAA injected in vivo (5.08-5.14 in P. pachytoides, and 6.22-6.74 for S. morganianum) were significantly greater than that of the other treatments. Since the h ormone-injected leaf cutting needs no aseptic operation which is necessary for in vitro multiplication, it is simple for the commercial production of the two species. The new in vivo propagation method would be of great interest for growers and breeders of succulent plants.

Author Comment

Our study established an efficient in vivo regeneration protocol by plant growth regulators injected leaves to meet the supply of young succulent ornamental plants of Pachyveria pachytoides. We believe the paper may be of particular interest to the readers of your journal as it provided a cheap in vivo propagation method, which would be useful for commercial production and breeding of succulent ornamental plants.