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Seasonal dynamics alters taxonomical and functional microbial profiles in Pampa biome soils under natural grasslands

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Seasonal dynamics alters taxonomical and functional microbial profiles in Pampa biome soils under natural grasslands https://t.co/kqrhhGxAQV #microbiomebot
211 days ago
Seasonal dynamics alters taxonomical and functional microbial profiles in Pampa biome soils under natural grasslands https://t.co/cPyTqvE4Gt
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Supplemental Information

Table S1. Location, soil taxonomy and soil physicochemistry analysis for two grasslands located in Santa Maria and São Gabriel municipalities

(1) Extracted with Mehlich-1. (2) Extracted with KCl 1 mol L-1. (3) Walkey & Black method. (4) Extracted with hot water. (5) Pipet method.

DOI: 10.7287/peerj.preprints.26569v1/supp-1

Table S2. Sequencing coverage for 16S rRNA (taxa) and mRNA (function) libraries derived from two grasslands located in Santa Maria and São Gabriel municipalities

SM = Santa Maria municipality; SG = São Gabriel municipality.

DOI: 10.7287/peerj.preprints.26569v1/supp-2

raw data figure 1

raw data figure 1

DOI: 10.7287/peerj.preprints.26569v1/supp-3

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Anthony Barboza conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Victor S Pylro conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Rodrigo Jacques conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Paulo Gubiani conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Júlio Trindade conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Eric Triplett conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Luiz Roesch conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

Raw sequences obtained by metatranscriptome sequencing and associated metadata were submitted to MG-RAST server (http://metagenomics.anl.gov/) and are public available under the string mgp12046. All raw sequences obtained by amplicon sequencing were submitted to NCBI Sequence Read Archive (SRA) and are available under the experiment number SRX2779549 run number SRR5499445.

Data Deposition

The following information was supplied regarding data availability:

The raw data/code is included in the manuscript (in the section 2.5. Bioinformatics analysis and statistics).

Funding

The authors acknowledge Dra. Manoeli Lupatini for taking soil samples from the Santa Maria site. We also like to thank the Fundação Estadual a Pesquisa Agropecuária do Estado do Rio Grande do Sul (FEPAGRO) for providing the experimental area for sampling in São Gabriel. This study was funded by the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Rio Grande do Sul (FAPERGS) and by the Brazilian Microbiome Project (http://www.brmicrobiome.org). VSP receives fellowship from FAPESP (Process number 2016/02219-8). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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