Seasonal dynamics alters taxonomical and functional microbial profiles in Pampa biome soils under natural grasslands

Anthony Barboza; Victor S Pylro; Rodrigo Jacques; Paulo Gubiani; Júlio Trindade; Eric Triplett; Luiz Roesch

doi:10.7287/peerj.preprints.26569v1

Seasonal dynamics alters taxonomical and functional microbial profiles in Pampa biome soils under natural grasslands

Anthony Barboza¹, Victor S Pylro ², Rodrigo Jacques³, Paulo Gubiani³, Júlio Trindade⁴, Eric Triplett⁵, Luiz Roesch¹

1 Centro Interdisciplinar de Pesquisas em Biotecnologia, Universidade Federal do Pampa, São Gabriel, Brazil

2 Soil Department, “Luiz de Queiroz” College of Agriculture, Piracicaba, Brazil

3 Soil Department, Universidade Federal de Santa Maria, Santa Maria, Brazil

4 Departamento de Diagnóstico e Pesquisa Agropecuária, Secretaria Estadual da Agricultura, Pecuária e Irrigação, São Gabriel, Brazil

5 Department of Microbiology and Cell Science, University of Florida, Gainesville, United States

DOI: 10.7287/peerj.preprints.26569v1

Published: 2018-02-23
Accepted: 2018-02-23

Subject Areas: Agricultural Science, Biodiversity, Bioinformatics, Ecology, Microbiology
Keywords: metatranscriptomics, 16S rRNA gene, abiotic factors, metataxonomics, seasons, soil microbial core, Ion Torrent PGM, NGS, subtropical ecosystems

Copyright: © 2018 Barboza et al.
Licence: This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.

Cite this article: Barboza A, Pylro VS, Jacques R, Gubiani P, Trindade J, Triplett E, Roesch L. 2018. Seasonal dynamics alters taxonomical and functional microbial profiles in Pampa biome soils under natural grasslands. PeerJ Preprints 6:e26569v1 https://doi.org/10.7287/peerj.preprints.26569v1

Abstract

Soil microbial communities’ assembly is strongly tied to changes in temperature and moisture. Although microbial functional redundancy seems to overcome taxonomical composition changes, the sensitivity and resilience of soil microbial communities from subtropical regions in response to seasonal variations are still poorly understood. Thus, the development of new strategies for biodiversity conservation and sustainable management require a complete understanding of the soil abiotic process involved in the selection of microbial taxa and functions. In this work, we used state of the art molecular methodologies (Next Generation Sequencing) to compare the taxonomic (metataxonomics) and functional (metatranscriptomics) profiles among soil samples from two subtropical natural grasslands located in the Pampa biome, Brazil, in response to short-term seasonal variations. We found consistent effects of season on both microbial community structure and functions, but with the former being more influenced than the latter. These variations were more related to the oscillation in the relative abundances of specific taxa along seasons, rather than extinction and recolonization of taxa along seasons. In conclusion, the most abundant microbial groups and functions were shared between seasons and locations reflecting the existence of a stable taxonomical and functional core microbiota.