Distribution of carbapenemases and efflux pump in carbopenems-resistance Acinetobacter baumannii

Department of Clinical Laboratory, Rushan Hospital of Binzhou Medical University, Rushan, Shandong, China
Department of respiration, Rushan Hospital of Binzhou Medical University, Rushan, Shandong, China
DOI
10.7287/peerj.preprints.2655v1
Subject Areas
Microbiology
Keywords
carbopenems-resistance Acinetobacter baumannii, efflux pump, carbapenemases, resistant mechanism
Copyright
© 2016 Qiu et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Qiu Z, Zhu L, Hou P. 2016. Distribution of carbapenemases and efflux pump in carbopenems-resistance Acinetobacter baumannii. PeerJ Preprints 4:e2655v1

Abstract

Acinetobacter baumannii has emerged as an important pathogen related to serious infections and nosocomial outbreaks around the world. The aim of this study was to detect the distribution of carbapenemases and efflux pump in carbopenems-resistance Acinetobacter baumannii(CRAB). In this study, 100 isolates of CRAB were collected from clinical specimens. Agar dilution was conducted to determine the minimum inhibitory concentrations (MICs) to 15 kinds of antibiotic. Genes of carbapenemases and efflux pumps were amplified by PCR. The expression difference of pump genes was also analyzed by real-time PCR between CRAB and carbopenems- sensitive Acinetobacter baumannii (CSAB). We found that most antibiotics, including aminoglycosides, fluoroquinolones and cephalosporins showed high MIC values in CRAB. While, all isolates were sensitive to polymyxin B. Among CRAB, 54, 32 and 16 isolates were positive for SHV-12,PER-1 and TEM-1, respectively. 86 isolates were positive for OXA-23. 55, 33 and 5 isolates carried adeB, adeJ and adeE genes. The expression level of adeB in CRAB was ten times higher than that in CSAB. Moreover, isolates with single adeE gene were detected for the first time in Acinetobacter baumannii.

Author Comment

This is a submission to PeerJ for review.

Supplemental Information

MIC values of 100 isolates of CRAB

DOI: 10.7287/peerj.preprints.2655v1/supp-1

Detection of genes amplication by PCR

DOI: 10.7287/peerj.preprints.2655v1/supp-2