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The interplay between movement, dispersal and morphology in Tetrahymena ciliates

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59 days ago
The interplay between movement, dispersal and morphology in Tetrahymena ciliates https://t.co/jI7sCDgivJ #protists https://t.co/wmxAh54Imo
214 days ago
The interplay between movement, dispersal and morphology in Tetrahymena ciliates https://t.co/xMhJJjiIw7
peerJ: The interplay between movement, dispersal and morphology in Tetrahymena ciliates https://t.co/my691uuiqU
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Supplemental Information

A two patch dispersal system made of two 1.5 mL microtubes connected by a silicon pipe, filled with nutritive medium, used to quantify dispersal rate from cells inoculated in the start patch and allowed to move freely during 6 h

DOI: 10.7287/peerj.preprints.26540v1/supp-1

An illustration of the raw trajectory data extracted from videos

Different colours show different individual trajectories. The linearity differed among trajectories with some being very linear and others more tortuous (see arrows). Some very short (in time or space) trajectories correspond to non-moving cells.

DOI: 10.7287/peerj.preprints.26540v1/supp-2

Relationships between movement metrics (speed and tortuosity) and cell morphology averaged at the genotype level (N=88)

Lines show the fit of the most parsimonious ANCOVA model relating cell morphology to movement metrics, considering variation due to the dispersal status. Larger cells moved faster and less tortuous and the effect was additive.

DOI: 10.7287/peerj.preprints.26540v1/supp-3

Relationships between relative changes in movement metrics (speed and tortuosity) and cell morphology (N=132)

Each point is the difference between the disperser and residents across genotypes and replicates. Lines show the fit of the most parsimonious ANCOVA model relating cell morphology to movement metrics. If disperser cells were relatively larger than residents, they moved faster. In contrast, if dispersers were more elongated than residents, they moved slower. Relative changes in size and shape did not influence the relative tortuosity.

DOI: 10.7287/peerj.preprints.26540v1/supp-4

Overview of the genotypes used in the experiment

DOI: 10.7287/peerj.preprints.26540v1/supp-5

Supplemental information

Details about genotypes used, the experimental system and the trajectory reconstruction.

DOI: 10.7287/peerj.preprints.26540v1/supp-6

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Frank Pennekamp conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Jean Clobert conceived and designed the experiments, contributed reagents/materials/analysis tools, authored or reviewed drafts of the paper, approved the final draft.

Nicolas Schtickzelle conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Data Deposition

The following information was supplied regarding data availability:

The data and code to reproduce the analyses presented in the manuscript can be found on figshare: https://figshare.com/s/cebfe4692d97bbaeee2d

Funding

FP was funded by Fonds Spéciaux de Recherche, Université catholique de Louvain. NS is a Research Associate of the Fund for Scientific Research (F.R.S.-FNRS). Financial support was provided by F.R.S.-FNRS and Université catholique de Louvain (ARC 10-15/031). Funding for JC is provided by the Laboratoire d’Excellence (LABEX) entitled TULIP (ANR-10-LABX-41). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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