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Candida albicans cell wall glycosidases DFG5 and DCW1 are required for biofilm formation and Hog-1 signaling

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214 days ago
Candida albicans cell wall glycosidases DFG5 and DCW1 are required for biofilm formation and Hog-1 signaling https://t.co/GM9tF5DZhg
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Supplemental Information

Full length Western Blot of P-Hog-1 for wildtype and parental strains.

DOI: 10.7287/peerj.preprints.26526v1/supp-1

Full length Western Blot of whole Hog-1 for DFG5/DCW1 mutant strains.

Lanes 1, 5 & 8: WT; Lanes 2, 6 & 9: ES1; Lanes 3, 7 & 10: ES195 (with Methionine & Cysteine). Lane 4: Protein Marker. Samples represent triplicates for ECL analysis of whole Hog-1.

DOI: 10.7287/peerj.preprints.26526v1/supp-2

Full length Western Blot ofP-Hog-1 for DFG5/DCW1 mutant strains

Lanes 1, 5 & 8: WT; Lanes 2, 6 & 9: ES1; Lanes 3, 7 & 10: ES195 (with Methionine & Cysteine). Lane 4: Protein Marker. Samples represent triplicates for ECL analysis of P-Hog-1.

DOI: 10.7287/peerj.preprints.26526v1/supp-3

Raw Data 1

The Raw Data 1 file is a docx format and includes the tables for the numeric data from Figures 1, 2, 3, 6B and 7B.

DOI: 10.7287/peerj.preprints.26526v1/supp-4

Raw Data 2

The Raw Data 2 file is in PDF format and includes the full length Wester Blots for Figures 6 and 7 and the information regarding antibodies used in this study.

DOI: 10.7287/peerj.preprints.26526v1/supp-5

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Ryan Mancuso conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Jennifer Chinnici conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Charlene Tsou conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Sujay Busarajan conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Abhiram Maddi conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, authored or reviewed drafts of the paper, approved the final draft.

Data Deposition

The following information was supplied regarding data availability:

The raw data is included in the supplemental figures.

Funding

The authors received no funding for this work.


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