Oligo-carrageenan (OC) kappa increases net photosynthesis, and basal and secondary metabolism enzyme activities in Eucalyptus globulus trees. Here, trees were sprayed on leaves with water (control) or with OC kappa 1 mg ml-1, once a week, four times in total, and cultivated for 17 additional weeks (21 weeks in total). Height, level of glucose, trehalose, TOR phosphorylated in Ser2448 (TOR-P) and transcripts encoding TOR and S6 kinase (S6K) as well as the level of transcripts encoding proteins and enzymes involved in glucose accumulation, photosynthesis, C, N and S assimilation, and synthesis of phenylpropanoid compounds (PPCs) and terpenes were determined. Treated trees showed an increase in height of 105% compared to controls at week 21. Treated trees showed an increase in glucose and trehalose level having an oscillatory pattern with maximal levels for glucose at week 1, 9-11 and 17-19, and for trehalose at weeks 1-3, 5, 8-9, 12, 15-16 and 18-21. TOR-P showed increases from week 1 until the end of the experiment with peaks at weeks 2, 6, 12 and 16. The level of tor transcripts showed peaks at weeks 3, 6, 10-11 and 13 whereas the level of s6k transcripts remained unchanged. In addition, transcripts encoding proteins involved in photosynthesis, and enzymes involved in glucose accumulation, C, N and S assimilation, and synthesis of secondary metabolites showed an oscillatory pattern with increases mainly at weeks 3-4, 5-6, 10-11, and in some cases at weeks 13-14 and 16-18. Thus, the increases in trehalose levels better correlate with increases in TOR-P and transcript levels. Therefore, OC kappa induced an increase in the levels of glucose, trehalose, TOR-P and expression of genes involved in photosynthesis, and basal and secondary metabolism which may explain, at least in part, the increase in growth and defense responses in E. globulus trees.