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Squeezing water from a stone: High-throughput sequencing from a 145-year old holotype resolves (barely) a cryptic species problem in flying lizards

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RT @GuinWogan: HTS sequencing from a Draco holotype https://t.co/hl9Ia6MXll via @PeerJPreprints
193 days ago
RT @GuinWogan: HTS sequencing from a Draco holotype https://t.co/hl9Ia6MXll via @PeerJPreprints
193 days ago
HTS sequencing from a Draco holotype https://t.co/hl9Ia6MXll via @PeerJPreprints
201 days ago
Squeezing water from a stone: High-throughput sequencing from a 145-year old holotype resolves (barely) a cryptic species problem in flying lizards https://t.co/7fapJE4jmU
Squeezing water from a stone: High-throughput sequencing from a 145-year old holotype resolves (barely) a cryptic species problem in flying lizards https://t.co/RpDq7JoWak
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Supplemental Information

ND2 sequence data for members of the Draco fimbriatus group plus 13 mitochondrial sequence reads obtained from the D. cristatellus holotype

This file includes ND2 sequence data obtained via traditional Sanger sequencing for 39 individuals representing Draco cristatellus, D. fimbriatus, D. hennigi, D. punctatus, and D. maculatus, plus 183 base pairs of ND2 obtained via MiSeq HTS for the Draco cristatellus holotype (BMNH 1872.2.19.4). In addition, the 13 mitochondrial sequence reads obtained for the D. cristatellus holotype are provided.

DOI: 10.7287/peerj.preprints.26466v1/supp-1

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Jimmy A McGuire conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables.

Darko D Cotoras conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, reviewed drafts of the paper.

Brendan O'Connell conceived and designed the experiments, performed the experiments, analyzed the data, reviewed drafts of the paper.

Shobi Z S Lawalata contributed reagents/materials/analysis tools.

Cynthia Y Wang-Claypool performed the experiments, reviewed drafts of the paper.

Alexander Stubbs analyzed the data, reviewed drafts of the paper.

Xiaoting Huang performed the experiments, reviewed drafts of the paper.

Guinevere O U Wogan performed the experiments, reviewed drafts of the paper.

Sarah M Hykin performed the experiments.

Sean B Reilly performed the experiments, reviewed drafts of the paper.

Ke Bi analyzed the data, contributed reagents/materials/analysis tools.

Awal Riyanto contributed reagents/materials/analysis tools.

Evy Arida reviewed drafts of the paper.

Lydia L Smith reviewed drafts of the paper.

Heather Milne performed the experiments.

Jeffrey W Streicher contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Djoko T Iskandar contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The University of California, Berkeley provided full approval of all Draco-related research undertaken as part of this project.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

Specimens collected in support of this research were obtained under research permits issued by the Economic Planning Unit (EPU) of Malaysia and the Indonesia Institute of Sciences (LIPI).

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

I will load the new Sanger sequence data onto GenBank if the paper is accepted. For review purposes, I loaded the data as a supplemental file. The raw MiSeq data will be loaded on to the Short Read Archive (SRA).

Data Deposition

The following information was supplied regarding data availability:

The research in this article did not generate any non-sequence data or code.

Funding

This work was supported by the National Geographic Society and the National Science Foundation (DEB 1652988). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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