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Supplemental Information

Effect plots from GLM on the experiment with adults exposed to low temperatures

The plots show the conditional coefficients (“marginal effects”) of all variables included in the model as well as effects resulting from the interaction terms. The variables are cold exposure temperature, duration, sex (Male vs Female) and all the interactions. The statistical outputs (from the table of deviance) are also given (in blue) before the plots for all terms of the model.

DOI: 10.7287/peerj.preprints.2646v1/supp-1

Effect plots from GLM on the experiment with pupae exposed to low temperatures

The plots show the conditional coefficients (“marginal effects”) of all variables included in the model as well as effects resulting from the interaction terms. The variables are cold exposure temperature, duration, and all the interactions. The statistical outputs (from the table of deviance) are also given (in blue) before the plots for all terms of the model.

DOI: 10.7287/peerj.preprints.2646v1/supp-2

Effect plots from GLM on the experiment with adults exposed to high temperatures

The plots show the conditional coefficients (“marginal effects”) of all variables included in the model as well as effects resulting from the interaction terms. The variables are heat exposure temperature, duration, sex (Male vs Female) and all the interactions. The statistical outputs (from the table of deviance) are also given (in blue) before the plots for all terms of the model.

DOI: 10.7287/peerj.preprints.2646v1/supp-3

Effect plots from GLM on the experiment with pupae exposed to high temperatures

The plots show the conditional coefficients (“marginal effects”) of all variables included in the model as well as effects resulting from the interaction terms. The variables are heat exposure temperature, duration and all the interactions. The statistical outputs (from the table of deviance) are also given (in blue) before the plots for all terms of the model.

DOI: 10.7287/peerj.preprints.2646v1/supp-4

Effect plots from GLM on the experiment with pupae exposed to low temperatures under two relative humidity levels

The plots show the conditional coefficients (“marginal effects”) of all variables included in the model as well as effects resulting from the interaction terms. The variables are cold exposure temperature, duration, RH and all the interactions. The statistical outputs (from the table of deviance) are also given (in blue) before the plots for all terms of the model.

DOI: 10.7287/peerj.preprints.2646v1/supp-5

Effect plots from GLM on the experiment with pupae exposed to high temperatures under two relative humidity levels

The plots show the conditional coefficients (“marginal effects”) of all variables included in the model as well as effects resulting from the interaction terms. The variables are heat exposure temperature, duration, RH and all the interactions. The statistical outputs (from the table of deviance) are also given (in blue) before the plots for all terms of the model.

DOI: 10.7287/peerj.preprints.2646v1/supp-6

Temperatures and respective exposure durations used for adult’s cold tolerance assays

DOI: 10.7287/peerj.preprints.2646v1/supp-7

Temperatures and respective exposure durations used for pupae’s cold tolerance assays

DOI: 10.7287/peerj.preprints.2646v1/supp-8

Temperatures and respective exposure durations used for adult’s heat tolerance assays

DOI: 10.7287/peerj.preprints.2646v1/supp-9

Temperatures and respective exposure durations used for pupae’s heat tolerance assays

DOI: 10.7287/peerj.preprints.2646v1/supp-10

Temperatures and respective exposure durations used for pupae’s thermal stress assays under high or low relative humidity

DOI: 10.7287/peerj.preprints.2646v1/supp-11

All dataset and scripts for reviewers only

DOI: 10.7287/peerj.preprints.2646v1/supp-12

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Thomas Enriquez conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Hercé Colinet conceived and designed the experiments, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Data Deposition

The following information was supplied regarding data availability:

Dropbox:

https://www.dropbox.com/sh/uoqaevj017ex6an/AABjOX4um0z_MUVqh42XtP61a?dl=0

Funding

This study was funded by SUZUKILL project (The French National Research Agency: ANR-15-CE21-0017 and Austrian Science Fund (FWF): I 2604-B25. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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