Morphohistological development of the somatic embryo of Typha domingensis
- Published
- Accepted
- Subject Areas
- Biotechnology, Developmental Biology, Plant Science, Histology, Freshwater Biology
- Keywords
- emerging aquatic macrophyte, sustainable propagation, somatic embryogenesis, embryogenic maturation, histodifferentiation
- Copyright
- © 2018 Hernández-Piedra et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2018. Morphohistological development of the somatic embryo of Typha domingensis. PeerJ Preprints 6:e26459v1 https://doi.org/10.7287/peerj.preprints.26459v1
Abstract
Background. The sustainable methods of propagation for Typha domingensis through somatic embryogenesis can help to mitigate its current condition of ecological marginalisation and overexploitation. Then, the hypothesis established that the variation of the concentration of auxin and light conditions in sequential stages of culture generate different morphogenetic routes that can be monitoring by morphohistological markers. Methods. Murashige and Skoog medium at half ionic strength, 3% sucrose and 0.1% ascorbic acid were used in the induction, proliferation and embryogenic maturation. Induction started with aseptic germinates cultured in 0.5 mg L-1 of 2,4-dichlorophenoxyacetic. Four concentrations of 0 to 2 mg L-1 of 2,4-dichlorophenoxyacetic, that generated four embryogenic lines, were evaluated in darkness. Maturation of the somatic embryo took place, in each embryogenic line, without auxin and under light and dark conditions. Results. The yellow and brown callus, as well as oblong and scutellar somatic embryos were recorded in the methodological sequence. The embryogenic differentiation was described with histological analysis. The induced cultures produced both somatic embryos in a small proportion. The percentages of the yellow callus on the explant and of suspended cells in the embryogenic proliferation were greater with the three concentrations of 2,4-dichlorophenoxyacetic. While, the brown callus predominated without auxin. The somatic embryo developed under light and dark conditions, and presented globular, oblong, scutellar and sparsely coleoptilar stages. Discussion. The combined effect of auxin concentrations and light-dark conditions generated conditions that favoured the development of embryogenic calluses and somatic embryos (globular, oblong, scutellar and coleoptilar) in an asynchronous process with respect to the stages of embryogenic induction, proliferation, and maturation. Indeed, differentiation and cellular organization of this process were compatible with descriptors of the embryogenic stages recorded by other aquatic and terrestrial monocotyledons.
Author Comment
This is a preprint submission to PeerJ Preprints.