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Immunogenicity evaluation of MS2 phage-mediated chimeric nanoparticle displaying an immunodominant B cell epitope of foot-and-mouth disease virus

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366 days ago
Immunogenicity evaluation of MS2 phage-mediated chimeric nanoparticle displaying an immunodominant B cell epitope of foot-and-mouth disease virus
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Supplemental Information

Raw data of ELISA to detect specific antibodies

Mice were immunized with PepVac, CNPs, TRE and PBS respectively. Mice sera were collected every week after immunization and used to detect the specific antibodies against inactivated FMDV by ELISA.

DOI: 10.7287/peerj.preprints.26448v1/supp-1

Raw data of lymphocyte proliferation assay

The T-lymphocyte proliferation of mice immunized with synthetic peptide vaccine, CNPs, TRE and PBS on 56 dpv. SI: OD450nm value of culture with FMDV stimulation/OD450nm value of culture without stimulation.

DOI: 10.7287/peerj.preprints.26448v1/supp-2

Raw data of cytokine levels

Splenic lymphocytes culture supernatants used in the proliferation assay were collected for evaluating IL-2, IL-4 and IFN-γ concentration. The assay were performed by the commercially available ELISA kit

DOI: 10.7287/peerj.preprints.26448v1/supp-3

Additional Information

Competing Interests

Juan Wang and Dongmin Liu are employees of Henan Zhongze Biological Engineering Co., Ltd. The authors declare that they have no competing interests.

Author Contributions

Guoqiang Wang performed the experiments, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Yunchao Liu conceived and designed the experiments.

Hua Feng contributed reagents/materials/analysis tools, wrote the paper.

Yumei Chen analyzed the data.

Suzhen Yang contributed reagents/materials/analysis tools.

Qiang Wei analyzed the data.

Juan Wang contributed reagents/materials/analysis tools.

Dongmin Liu contributed reagents/materials/analysis tools.

Gaiping Zhang conceived and designed the experiments.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The Animal Experiment Committee of Henan Academy of Agricultural Sciences


This work was supported by grants from National Key R&D Program (2017YFD0501103), Henan province science and technology key project (142102110158), and Henan Province basic and frontier technology research project (152300410238). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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