Repetitive low intensity magnetic field stimulation in a neuronal cell line: A metabolomics study

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1 School of Veterinary and Life Sciences, Murdoch University, Murdoch, WA, Australia
2 School of Biological Sciences, Experimental and Regenerative Neuroscience, The University of Western Australia, Crawley, WA, Australia
3 Brain Plasticity laboratory, Perron Institute for Translational Neuroscience, Perth, WA, Australia
DOI
10.7287/peerj.preprints.26446v1
Published
Accepted
Subject Areas
Biochemistry
Keywords
rTMS, ELF-MF, GABA, neuron
Copyright
© 2018 Hong et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Hong I, Garrett A, Maker G, Mullaney I, Rodger J, Etherington SJ. 2018. Repetitive low intensity magnetic field stimulation in a neuronal cell line: A metabolomics study. PeerJ Preprints 6:e26446v1

Abstract

Low intensity repetitive magnetic stimulation of neural tissue modulates neuronal excitability and has promising therapeutic potential in the treatment of neurological disorders. However, the underpinning cellular and biochemical mechanisms remain poorly understood. This study investigates the behavioural effects of low intensity repetitive magnetic stimulation (LI-rMS) at a cellular and biochemical level. We delivered LI-rMS (10 mT) at 1 Hz and 10 Hz (n=5 wells per group) to B50 rat neuroblastoma cells in vitro for 10 minutes and measured levels of selected metabolites immediately after stimulation. LI-rMS at both frequencies depleted selected tricarboxylic acid (TCA) cycle metabolites without affecting the main energy supplies. Furthermore, LI-rMS effects were frequency-specific with 1 Hz stimulation having stronger effects than 10 Hz. The observed depletion of metabolites was consistent with an increase in GABA release as a result of higher spontaneous activity. Although the absence of organised neural circuits and other cellular contributors (e.g. excitatory neurons and glia) in the B50 cell line limits the degree to which our results can be extrapolated to the human brain, the changes we describe provide novel insights into how LI-rMS modulates neural tissue.

Author Comment

This is a submission to PeerJ for review.

Supplemental Information

Raw data for metabolite levels

Sheet 1: raw values

Sheet 2: normalised values

DOI: 10.7287/peerj.preprints.26446v1/supp-1