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Supplemental Information

Table S1. Extended summary of genome bins

DOI: 10.7287/peerj.preprints.2613v1/supp-1

Table S2. Summary of SSU rRNA genes in genome bins

DOI: 10.7287/peerj.preprints.2613v1/supp-2

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Sean P Jungbluth conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Jan P Amend reviewed drafts of the paper.

Michael S Rappé conceived and designed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The U1362A and U1362B metagenome projects and raw sequencing reads are available via the IMG-M web portal under Taxon ID numbers 330002481 (U1362A) and 3300002532 (U1362B). Gold Analysis Project ID numbers are Ga0004278 (U1362A) and Ga0004277 (U1362B). Sample metadata can be accessed using the BioProject identifier PRJNA269163. The NCBI BioSamples used here are SAMN03166137 (U1362A) and SAMN03166138 (U1362B). FASTA files containing the contigs of all 98 genomes from metagenomes can be accessed at doi: 10.6084/m9.figshare.4269587.v1. A FASTA file containing 54 SSU rRNA genes with length >300 base pairs extracted from the 98 genomes from metagenomes can be accessed at doi: 10.6084/m9.figshare.4269593.v1.

Data Deposition

The following information was supplied regarding data availability:

IMG/M-relevant files needed to isolate scaffold sets for all 98 genomes from metagenomes can be accessed at doi: 10.6084/m9.figshare.4269590.v1.

IMG/M annotations associated with the contigs of all 98 genomes from metagenomes can be accessed at doi: 10.6084/m9.figshare.4269581.v1.

Funding

This work, including the efforts of Sean Jungbluth and Michael Rappé, was funded by National Science Foundation grants MCB06-04014 and OCE-1260723. This work, including the efforts of all authors, was supported by the Center for Dark Energy Biosphere Investigations (C-DEBI), a National Science Foundation-funded Science and Technology Center of Excellence (OCE-0939564). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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