Validation and characterization of thirteen microsatellite markers for queen conch, Lobatus gigas

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1 Smithsonian Marine Station, Smithsonian National Museum of Natural History, Fort Pierce, Florida, USA
2 School of Science and the Environment, The Manchester Metropolitan University, Manchester, United Kingdom
DOI
10.7287/peerj.preprints.2559v1
Published
Accepted
Subject Areas
Bioengineering, Bioinformatics, Conservation Biology, Genetics, Genomics
Keywords
conservation, connectivity, marine gastropod
Copyright
© 2016 Truelove et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Truelove NK, Fai Ho L, Preziosi RF, Box SJ. 2016. Validation and characterization of thirteen microsatellite markers for queen conch, Lobatus gigas. PeerJ Preprints 4:e2559v1

Abstract

We report the development and characterization of 13 novel microsatellite loci for the Caribbean queen conch, Lobatus gigas, an ecologically and commercially important marine gastropod. Paired-end sequencing was carried out on genomic DNA from a single queen conch using half a flow cell lane of an Illumina MiSeq. A total of 48 potentially amplifiable loci containing microsatellites were tested on 45 individuals from the Florida Keys and Bahamas. In total, 13 consistently amplifying and polymorphic microsatellite loci were identified. The number of alleles ranged from 4 to 26 and observed heterozygosities ranged from 0.340 to 1.00. There was no evidence of scoring error, large allele dropout, or evidence of linkage disequilibrium at any locus. Four loci deviated from Hardy-Weinberg equilibrium due to moderate levels of null alleles (null allele frequencies ranged from 0.081 to 0.230). Although null alleles were detected at four microsatellite loci, the high levels of polymorphism and moderate null allele frequencies suggest that these 13 novel microsatellite markers will be useful for researchers carrying out conservation genetic studies of L. gigas.

Author Comment

This is a preprint submission to PeerJ Preprints.

Supplemental Information

Dataset of microsatellite genotypes used in Table 1

Text file of microsatellite genotypes used in Table 1 in the Genepop format

DOI: 10.7287/peerj.preprints.2559v1/supp-1