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Von Hagen RL, Khan RA, Woltmann S.2016. Fifteen novel microsatellites for the Louisiana Waterthrush (Aves: Parulidae: Parkesia motacilla) using MiSeq sequencing. PeerJ Preprints4:e2498v1https://doi.org/10.7287/peerj.preprints.2498v1
The Louisiana Waterthrush (Parkesia motacilla) breeds along wooded streams throughout much of eastern North America, and winters in the Caribbean and Central America. Because of its dependence on stream macroinvertebrates—which are themselves dependent on high water quality—the Louisiana Waterthrush may serve as a useful bioindicator of both stream and landscape integrity. Perhaps unique among eastern North American songbirds, the Louisiana Waterthrush often maintains essentially linear territories along streams, and this provides a unique context in which to ask questions about the genetic (as opposed to social) mating system of this species. We developed 15 microsatellite loci for Louisiana Waterthrush using MiSeq sequencing. All loci presented here are polymorphic, with 3-15 alleles detected in a reference sample of 35-43 individuals. For parentage analyses, these loci have a combined non-exclusion probability of 0.0011 if neither parent is known a priori, and a non-exclusion probability of < 0.0001 if one parent is known. These 15 loci thus provide high discriminatory power to assign parentage to nestlings, and can also be used to examine population genetic structure within the species.
This is a microsatellite primer note describing 15 loci developed specifically for the Louisiana Waterthrush (Parkesia motacilla), using MiSeq for initial sequence discovery. This suite of loci will be useful in addressing questions of parentage analyses, and is also suitable for examining range-wide population structure.
File S1: Genotypes (in GenePop format) of individuals used to test these microsatellite loci