TY - JOUR UR - https://doi.org/10.7287/peerj.preprints.2498v1 DO - 10.7287/peerj.preprints.2498v1 TI - Fifteen novel microsatellites for the Louisiana Waterthrush (Aves: Parulidae: Parkesia motacilla) using MiSeq sequencing AU - Von Hagen,R. Lynn AU - Khan,Rafia A AU - Woltmann,Stefan DA - 2016/10/03 PY - 2016 KW - microsatellites KW - birds KW - Parulidae AB - The Louisiana Waterthrush (Parkesia motacilla) breeds along wooded streams throughout much of eastern North America, and winters in the Caribbean and Central America. Because of its dependence on stream macroinvertebrates—which are themselves dependent on high water quality—the Louisiana Waterthrush may serve as a useful bioindicator of both stream and landscape integrity. Perhaps unique among eastern North American songbirds, the Louisiana Waterthrush often maintains essentially linear territories along streams, and this provides a unique context in which to ask questions about the genetic (as opposed to social) mating system of this species. We developed 15 microsatellite loci for Louisiana Waterthrush using MiSeq sequencing. All loci presented here are polymorphic, with 3-15 alleles detected in a reference sample of 35-43 individuals. For parentage analyses, these loci have a combined non-exclusion probability of 0.0011 if neither parent is known a priori, and a non-exclusion probability of < 0.0001 if one parent is known. These 15 loci thus provide high discriminatory power to assign parentage to nestlings, and can also be used to examine population genetic structure within the species. VL - 4 SP - e2498v1 T2 - PeerJ Preprints JO - PeerJ Preprints J2 - PeerJ Preprints SN - 2167-9843 ER -