Fifteen novel microsatellites for the Louisiana Waterthrush (Aves: Parulidae: Parkesia motacilla) using MiSeq sequencing

Biology Department and Center of Excellence for Field Biology, Austin Peay State University, Clarksville, Tennessee, United States
Department of Biology, Western Kentucky University, Bowling Green, Kentucky, United States
Department of Entomology and Nematology, University of Florida, Gainesville, Florida, United States
DOI
10.7287/peerj.preprints.2498v1
Subject Areas
Ecology, Genetics, Molecular Biology, Zoology
Keywords
microsatellites, birds, Parulidae
Copyright
© 2016 Von Hagen et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Von Hagen RL, Khan RA, Woltmann S. 2016. Fifteen novel microsatellites for the Louisiana Waterthrush (Aves: Parulidae: Parkesia motacilla) using MiSeq sequencing. PeerJ Preprints 4:e2498v1

Abstract

The Louisiana Waterthrush (Parkesia motacilla) breeds along wooded streams throughout much of eastern North America, and winters in the Caribbean and Central America. Because of its dependence on stream macroinvertebrates—which are themselves dependent on high water quality—the Louisiana Waterthrush may serve as a useful bioindicator of both stream and landscape integrity. Perhaps unique among eastern North American songbirds, the Louisiana Waterthrush often maintains essentially linear territories along streams, and this provides a unique context in which to ask questions about the genetic (as opposed to social) mating system of this species. We developed 15 microsatellite loci for Louisiana Waterthrush using MiSeq sequencing. All loci presented here are polymorphic, with 3-15 alleles detected in a reference sample of 35-43 individuals. For parentage analyses, these loci have a combined non-exclusion probability of 0.0011 if neither parent is known a priori, and a non-exclusion probability of < 0.0001 if one parent is known. These 15 loci thus provide high discriminatory power to assign parentage to nestlings, and can also be used to examine population genetic structure within the species.

Author Comment

This is a microsatellite primer note describing 15 loci developed specifically for the Louisiana Waterthrush (Parkesia motacilla), using MiSeq for initial sequence discovery. This suite of loci will be useful in addressing questions of parentage analyses, and is also suitable for examining range-wide population structure.

Supplemental Information

File S1: Genotypes (in GenePop format) of individuals used to test these microsatellite loci

DOI: 10.7287/peerj.preprints.2498v1/supp-1