We have added a detailed description of what motivated the concept of cryoelectrolysis. The description is supported by a new figure (Fig1), which draws from the experience in the field of one of the authors (BR). Please see lines 144 to 213 and Fig.1
We have added a section to the introduction that emphasizes the difference between our previous study on cryoelectrolysis and this one. In particular, we introduced the new hypothesis that is examine in this study and the potential clinical value. Please see lines 222 to 253 in the introduction.
The justification for the choice of the currents was missing. Essentially, we chose typical values used in electrolytic ablation processes. In addition, we have done preliminary experiments only with electrolysis to identify current values that will provide us with measurable results in our configuration. Please see lines 320 to 327 in the experimental protocol.
We substantially changed the entire introduction. The difference between our earlier work and this one is explained in detail. In the previous work we delivered first electrolysis and followed with freezing. In this study we delivered electrolysis and freezing/thawing simultaneously. Please see the revised introduction. <!--[if !supportLineBreakNewLine]-->
Former Figure 1, was separated now into Fig 2 and Fig 3. Fig 2 that is relevant to materials and methods was put in the relevant section and Fig 3 was moved to results and Discussion.
We have reorganized the results and discussion into groups, to make them easier to follow and understand.
We have added details on the fundamental scientific principles of this work in both, the introduction and the results and discussion section. The details are supported by two new figures, 1 and 6. Please see the relevant additions on lines 144 to 213 and Fig.1 and lines 747 to 756 and Fig.6.