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Supplemental Information

Dataset and R script used for the statistical analyses

DOI: 10.7287/peerj.preprints.2458v1/supp-1

Detailed characteristics of each sampling site

DOI: 10.7287/peerj.preprints.2458v1/supp-2

Bray-Curtis dissimilarity of foliar fungal communities between each pair of host species and for each sampling date

These values were calculated exclusively with dissimilarity between samples within sampling sites (mean ± SD).

DOI: 10.7287/peerj.preprints.2458v1/supp-3

Results of PERMANOVA for OTU composition for both foliar and airborne fungal communities

The response variables are averaged Jaccard dissimilarity matrices, computed over 100 rarefactions of the OTU matrix. In both models, sampling site was included as a stratification variable. The values in bold are significant.

DOI: 10.7287/peerj.preprints.2458v1/supp-4

OTU richness of foliar fungal communities for all host species, depending on the sampling date

Oak fungal foliar community richness is represented in green, hornbeam in blue, chestnut in orange and grapevine in red. Error bars represent the SE of the mean. Different letters indicate significant differences in mean (Tukey's post-hoc pairwise comparison test).

DOI: 10.7287/peerj.preprints.2458v1/supp-5

Edge effect on the OTU richness of foliar fungal communities for all host species

Error bars represent the SE of the mean.

DOI: 10.7287/peerj.preprints.2458v1/supp-6

NMDS representing foliar fungal community dissimilarities

(a) Bray-Curtis dissimilarities, computed over 100 random rarefaction matrices. The stress value associated with these representations was 0.170. (b) Jaccard dissimilarities, computed over 100 random rarefaction matrices. The stress value associated with these representations was 0.158. Each point represents a sample: colours indicate the host species (hornbeam in blue, chestnut in orange, oak in green and grapevine in red) and the shape of the symbol indicates the sampling date. The confidence ellipsoid at the 0.68 level is shown for all combinations of these two factors.

DOI: 10.7287/peerj.preprints.2458v1/supp-7

ANOVA table performed on the richness calculated with the unrarefied OTU table

Effect of the abundance (number of sequences per sample), sampling date (May, July or October), host species (oak, hornbeam, chestnut or grapevine) or habitat (vineyard or forest), edge (habitat centre or center) and their interaction on OTU richness in foliar and airborne fungal communities. In both models, sampling site was included as a random variable. Rm2 is the marginal coefficient of determination (for fixed effects) and Rc2 the conditional coefficient of determination (for fixed and random effects). Bold values ares the significant ones.

DOI: 10.7287/peerj.preprints.2458v1/supp-8

PERMANOVA table, performed on the Bray-Curtis dissimilarity matrix calculated with the unrarefied OTU matrix

Effect of sampling date (May, July or October), host species (oak, hornbeam, chestnut or grapevine) or habitat (vineyard or forest), edge (habitat centre or center) and their interaction on the composition of foliar and airborne fungal communities. In both models, sampling site was included as a stratification variable. Bold values ares the significant ones.

DOI: 10.7287/peerj.preprints.2458v1/supp-9

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Thomas Fort performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Cécile Robin conceived and designed the experiments, performed the experiments, wrote the paper, reviewed drafts of the paper.

Xavier Capdevielle performed the experiments.

Laurent Delière conceived and designed the experiments, performed the experiments, reviewed drafts of the paper.

Corinne Vacher conceived and designed the experiments, performed the experiments, wrote the paper, reviewed drafts of the paper.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

European Nucletide Archive.

Project accession number : PRJEB13880

Data Deposition

The following information was supplied regarding data availability:

The raw data has been supplied as a supplementary file.

Funding

This study was carried out with financial support from the French National Research Agency (ANR) in the frame of the Investments for the future Programme, within the COTE Cluster of Excellence (ANR-10-LABX-45). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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