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Supplemental Information

Length and counts of the harbor seal transcriptome

(A) The length and count distribution of the harbor seal transcriptome with transcript lengths < 8,500 and (B) the length and count distribution of the harbor seal transcriptome with transcript lengths > 8,500.

DOI: 10.7287/peerj.preprints.2457v1/supp-1

Significant differentially expressed genes (DEGs, padj < 0.05) that fell within GO category “defense response to bacteria.”

Heatmap hierarchical clustering of normalized gene counts expressed in rlog transformation (row z-score) from PhV1com and UCD harbor seals.

DOI: 10.7287/peerj.preprints.2457v1/supp-2

Transcriptome assemblies conducted using quality controlled sequence reads from both libraries (UCD and PhV-1com)

DOI: 10.7287/peerj.preprints.2457v1/supp-3

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Stephanie M Rosales conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables.

Rebecca L Vega Thurber contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

This work was authorized by the National Marine Fisheries (NMFS) for possession of tissue samples from stranded marine mammals. This work is in compliance with the Marine Mammal Protection Act (MMPA) regulation 50 CR 216.22 and 216.37.

Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

This work was authorized by the National Marine Fisheries (NMFS) for possession of tissue samples from stranded marine mammals. This work is in compliance with the Marine Mammal Protection Act (MMPA) regulation 50 CR 216.22 and 216.37.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

Figshare

https://dx.doi.org/10.6084/m9.figshare.3581712.v1

Data Deposition

The following information was supplied regarding data availability:

The raw sequence data supporting the conclusions of this article is available in the European Nucleotide Archive repository, using accession number: PRJEB11686 (http://www.ebi.ac.uk/ena/data/view/PRJEB11686)

Funding

This work was supported by Sea Grant Starter Grant # NA010AR4170059 NA223B R700 to RVT and an NSF Graduate Research Fellowship #2012136295 to SMR. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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