Visitors   Views   Downloads
NOT PEER-REVIEWED
"PeerJ Preprints" is a venue for early communication or feedback before peer review. Data may be preliminary.

Supplemental Information

NeuN and GFAP staining in the cerebellum at P7 of tgFgfr1-EGFP+ mice

NeuN+ granule cell layer neurons did not colocalize with GFP at P7 (A, low magnification, B, high magnification). GFAP+ glia of the cerebellum do colocalize with GFP at P7 (C, low magnification, D, high magnification). Arrow heads denote double labeled cells while small arrows denote GFAP+/GFP+ glial fibers. Scale bar is 50µm in A, C, and 25µm in B, D.

DOI: 10.7287/peerj.preprints.2343v1/supp-1

Fgfr1 promoter driven GFP does not colocalize with Olig2+ cells in the hippocampus

Olig2 staining in the hippocampus of control tgfgfr1-EGFP- mice ( A-D) and tgfgfr1-EGFP+ mice (E-H). Scale bar is 50µm.

DOI: 10.7287/peerj.preprints.2343v1/supp-2

Fgfr1 expression in the hypothalamus does not colocalize with OLIG2 and does have some colocalization with BLBP

Fgfr1 promoter driven GFP in the hypothalamus of control tgfgfr1-EGFP- mice (A,B) and tgfgfr1-EGFP+ mice (C-F). OLIG2+ cells in the hypothalamus do not colocalize with Fgfr1 promoter driven GFP+ (C, D). BLBP+ cells near the third ventricle do colocolize with Fgfr1 promoter driven GFP+ (E), but there were not many of these colocalized cells in the medial eminence (F). Scale bar is 50µm in A, C, E and 25µm in B, D, F.

DOI: 10.7287/peerj.preprints.2343v1/supp-3

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Lisha Choubey conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Jantzen C Collette performed the experiments, prepared figures and/or tables, reviewed drafts of the paper.

Karen Muller Smith conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

University of Louisiana at Lafayette IACUC committee APS number 2012-8717-046, 2013- 8717-053, 2014-8717 040, 2015-8717-033

Data Deposition

The following information was supplied regarding data availability:

https://figshare.com/s/d16b14dd2e71525a7293

https://figshare.com/s/36bc336d5b0ccb95efe8

https://figshare.com/s/722ecee87a3f32427759

https://figshare.com/s/f17d6889939bd680152d

Funding

This work was supported by the Brain Behavior Research Foundation Young Investigator Award (KMS), the National Institute of Mental Health (K01MH087845), Ray P. Authement College of Sciences start up funds from the University of Louisiana at Lafayette (KMS), and the Graduate Student Organization at the University of Louisiana at Lafayette (LC). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


Add your feedback

Before adding feedback, consider if it can be asked as a question instead, and if so then use the Question tab. Pointing out typos is fine, but authors are encouraged to accept only substantially helpful feedback.

Some Markdown syntax is allowed: _italic_ **bold** ^superscript^ ~subscript~ %%blockquote%% [link text](link URL)
 
By posting this you agree to PeerJ's commenting policies