Whole-brain ex-vivo quantitative MRI of the cuprizone mouse

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1 Department of Neuroimaging, IOPPN, King's College London, London, United Kingdom
2 Centre for Functional MRI of the Brain, University of Oxford, Oxford, Oxfordshire, United Kingdom
3 Synaptive Medical, Toronto, ON, Canada
4 Cells and Behaviour Unit, Department of Basic and Clinical Neuroscience, IOPPN, King's College London, London, United Kingdom
5 NatBrainLab, Department of Basic and Clinical Neuroscience, IOPPN, King's College London, London, United Kingdom
DOI
10.7287/peerj.preprints.2323v1
Published
Accepted
Subject Areas
Neuroscience, Radiology and Medical Imaging, Histology
Keywords
cuprizone, myelin, quantitative imaging, MRI
Copyright
© 2016 Wood et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Wood TC, Simmons C, Hurley SA, Vernon AC, Torres J, Dell'Acqua F, Williams SCR, Cash D. 2016. Whole-brain ex-vivo quantitative MRI of the cuprizone mouse. PeerJ Preprints 4:e2323v1

Abstract

Myelin is a critical component of the nervous system and a major contributor to contrast in Magnetic Resonance (MR) images. However the precise contribution of myelination to multiple MR modalities is still under debate. The cuprizone mouse is a well established model of demyelination that has been used in several MR studies, but these have often imaged only a single slice and analysed a small region of interest in the corpus callosum. We imaged and analyzed the whole brain of the cuprizone mouse ex-vivo using high-resolution quantitative MR methods (multi-component DESPOT, Diffusion Tensor Imaging and Tensor Based Morphometry) and found changes in multiple regions, including the corpus callosum, cerebellum, thalamus and hippocampus. However the presence of inflammation, confirmed with histology, presents difficulties in isolating the sensitivity and specificity of these MR methods to demyelination using this model.

Author Comment

This is a submission to PeerJ for review.