Null and empirical distributions of the association score in ST-45 clonal complex.
Distribution of squared correlation coefficient (linkage disequilibrium r2) values between SNPs across C. jejuni genomic regions longer than 100 kb. (A) Core and (B) disease-associated SNPs with 25% minor allele frequency are used for calculation.
Clinical strains from ST-21 clonal complex analysed in this study. Clinical strains used for discovery and replication in GWAS are coloured in red and pink, respectively. The tree was constructed from the core genes in ST-21 clonal complex by using FastTree.
Sensitivity of C. jejuni NCTC11168 and nucleotide salvage mutants to toxic nucleotide analogues. (A) C. jejuni NCTC11168 wildtype was assessed for its sensitivity to the toxic nucleotide analogues AZ (8-Azaxanthine monohydrate), DP (2,6-Diaminopurine), FU (5-Fluorouracil), MP (6-Mercaptopurine) and TG (6-Thioguanine) with a DMSO control. Of those tested, sensitivity was only seen towards MP and TG. * p<0.05, *** p<0.001 (B) Disc diffusion assays were used to evaluate the resistance of nucleotide salvage mutants cj1368-70 to 6-Mercaptopurine (MP) and 6-Thioguanine (TP). Each single mutant, and a triple mutant, were completely resistant compared to wildtype.
Prevalence of associated words in Campylobacter genomes from various sources. (A) Prevalence on 354 genomes from cattle (n=43) chicken (n=300) and wild birds (n=11). Genomes have been previously published or are part of this study. (B) Prevalence on the 209 clinical genomes sampled before 2010, in 2010, and in 2011.
Description of sequenced isolates used in this study.
Primers and vectors used for mutant construction in this study.
Summary of the classification of the survival-associated words.
Allelic types and predicted protein variants of the nuoK gene in 44 ST-21 clonal complex and 37 ST-45 clonal complex isolates used in the GWAS and phenotypical testing.