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Thomas NC, Schwessinger B, Liu F, Chen H, Wei T, Nguyen YP, Shaker IW, Ronald PC. (2016) XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves. PeerJ Preprints4:e2289v1https://doi.org/10.7287/peerj.preprints.2289v1
The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo). We developed a detached leaf infection assay to quickly and reliably measure activation of the XA21-mediated immune response using genetic markers. We used RNA sequencing of elf18 treated EFR:XA21:GFP plants to identify candidate genes that could serve as markers for XA21 activation. From this analysis we identified 8 genes that are up-regulated in both in elf18 treated EFR:XA21:GFP rice leaves and Xoo infected XA21 rice leaves. These results provide a rapid and reliable method to assess bacterial-rice interactions.
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RNA sequencing sample treatment summary
Table summarizes the experimental setup including the genotypes, time of treatment, and type of treatment used for samples used in RNA sequencing. There were three replicates for each sample for a total of 21 sequenced samples.
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