XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves
- Published
- Accepted
- Subject Areas
- Agricultural Science, Plant Science
- Keywords
- XA21, Xanthomonas, EFR, Rice, RNAseq
- Copyright
- © 2016 Thomas et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2016. XA21-specific induction of stress-related genes following Xanthomonas infection of detached rice leaves. PeerJ Preprints 4:e2289v1 https://doi.org/10.7287/peerj.preprints.2289v1
Abstract
The rice XA21 receptor kinase confers robust resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo). We developed a detached leaf infection assay to quickly and reliably measure activation of the XA21-mediated immune response using genetic markers. We used RNA sequencing of elf18 treated EFR:XA21:GFP plants to identify candidate genes that could serve as markers for XA21 activation. From this analysis we identified 8 genes that are up-regulated in both in elf18 treated EFR:XA21:GFP rice leaves and Xoo infected XA21 rice leaves. These results provide a rapid and reliable method to assess bacterial-rice interactions.
Author Comment
This is a preprint submission to PeerJ Preprints.
Supplemental Information
RNA sequencing sample treatment summary
Table summarizes the experimental setup including the genotypes, time of treatment, and type of treatment used for samples used in RNA sequencing. There were three replicates for each sample for a total of 21 sequenced samples.