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Müller-Moulé P, Nozue K, Pytlak ML, Palmer CM, Covington MF, Wallace AD, Harmer SL, Maloof JN. (2016) YUCCA auxin biosynthetic genes are required for Arabidopsis shade avoidance. PeerJ Preprints4:e2211v2https://doi.org/10.7287/peerj.preprints.2211v2
Plants respond to neighbor shade by increasing stem and petiole elongation. Shade, sensed by phytochrome photoreceptors, causes stabilization of PHYTOCHROME INTERACTING FACTOR proteins and subsequent induction of YUCCA auxin biosynthetic genes. To investigate the role of YUCCA genes in phytochrome-mediated elongation we examined auxin signaling kinetics after an end-of-day far-red (EOD-FR) light treatment, and found that an auxin responsive reporter is rapidly induced within 2 hours of far-red exposure. YUCCA2, 5, 8, and 9 are all induced with similar kinetics suggesting that theycould act redundantly to control shade-mediated elongation. To test this hypothesis we constructed a yucca2,5,8,9 quadruple mutant and found that the hypocotyl and petiole EOD-FR and shade avoidance are completely disrupted. This work shows that YUCCA auxin biosynthetic genes are essential for detectable shade avoidance and that YUCCA genes are important for petiole shade avoidance.
This version changes in response to reviewer comments at PeerJ. The manuscript has been updated to more clearly describe experimental conditions. We also better distinguish between end-of-day far-red (EOD-FR) and low red:fr (R:FR) treatments and fixed a number of minor errors.
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