Effect of body condition on follicle transcriptome in estrous goats
- Published
- Accepted
- Subject Areas
- Developmental Biology, Genomics
- Keywords
- body condition, gene, follicle, transcriptome, goat, pathway
- Copyright
- © 2016 Wang et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2016. Effect of body condition on follicle transcriptome in estrous goats. PeerJ Preprints 4:e2177v1 https://doi.org/10.7287/peerj.preprints.2177v1
Abstract
Body condition or obesity has profound effects on estrus, follicle development, endocrine and conception in both livestock and human beings. To identify potential genes and pathways associated with follicle development in goats of different body condition scores (BCSs), the global gene expression levels of 2.0-3.5 mm follicles from 24 Yangtze River Delta white goats were analyzed by ribonucleic acid sequencing (RNA-Seq). A total of 2,019 differentially expressed genes (DEGs) were identified in obese goats compared with the control, with 381 up-regulated and 1,638 down-regulated. A total of 309 DEGs were associated with reproduction in top-15-cluster-frequency Gene Ontology (GO) terms, and they accounted for 47.3% of all DEGs in biological process classification (653). Using Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis, several pathways relevant to reproduction and metabolism were enriched, such as cytokine-cytokine receptor interaction, steroid hormone biosynthesis, etc. Based on the results of GO analysis and KEGG analysis, eight DEGs related to reproduction and metabolism were selected for quantitative real-time polymerase chain reaction (qRT-PCR) assays. The results showed that the expression level of these genes exhibited similar trends to the expression pattern of RNA-Seq. In addition, no significant difference was found in the intrafollicular estradiol (E2) concentration between the obese group and the control. The concentrations of progesterone (P4) and leptin were lower, and a higher E2/P4 ratio was found in the obese group. In summary, the establishment of goat follicle transcriptome would provide a useful reference for the molecular mechanism of follicle development. The combined analysis of DEGs, enriched pathways and follicular fluid composition revealed that P4 and leptin might be potential biomarkers of follicle development in goats.
Author Comment
This is a submission to PeerJ for review.
Supplemental Information
Summary of Reads and gene number of follicles from goats of different BCS
Differentially regulated genes of follicles between obese and normal goats
Screening criteria for DEGs: FDR (false discovery rate) < 0.05 and |log2FC|>1