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Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Alan J Couch conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Peter J Unmack conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Fiona J Dyer conceived and designed the experiments, reviewed drafts of the paper.

Mark Lintermans conceived and designed the experiments, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

1. University of Canberra Committee for Ethics in Animal Experimentation.

2. Research was conducted under approvals CEAE 11-15 and CEAE 13-17

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

Fish were collected under ACT Government licences LT2011516, LT2012590 and LT20133653.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

Genbank accession numbers:

KX355263

KX355264

KX355265

KX355266

KX355267

KX355268

KX355269

KX355270

KX355271

KX355272

KX355273

KX355274

Data Deposition

The following information was supplied regarding data availability:

Github: https://github.com/dnatheist/Maccullochella-hybridisation

Funding

The Australian Government Department of Education and Training and ICON Water (ACT) provided funding for this project as a PhD student scholarship. Additional financial support was provided by Carlton-Mid through Recreational Fishing Research (recfishingresearch.org) The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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