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Supplemental Information

S1 Supplementary Methods

Details of the analysis pipeline used and an outline of each clustering method.

DOI: 10.7287/peerj.preprints.2115v1/supp-1

S2 Supplementary Table

Summary of OTU counts resulting from each method

DOI: 10.7287/peerj.preprints.2115v1/supp-2

S3 Supplementary Table

Method-wise A,C and E Estimates for Each Taxa Found Across All Methods

DOI: 10.7287/peerj.preprints.2115v1/supp-3

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Matthew A Jackson conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Jordana T Bell contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Tim D Spector contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Claire J Steves contributed reagents/materials/analysis tools, reviewed drafts of the paper, supervised all work carried out by Matthew A Jackson.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Ethical approval for microbiota studies within TwinsUK were provided by the NRES Committee London – Westminster (REC Reference No.: EC04/015).

Data Deposition

The following information was supplied regarding data availability:

Sequencing data used within these experiments is available as part of the European Nucleotide Archive (ENA) study with the accession ERP015317.


The TwinsUK microbiota project was funded the National Institutes of Health (NIH) RO1 DK093595, DP2 OD007444. TwinsUK received funding from the Wellcome Trust; European Community’s Seventh Framework Programme (FP7/2007-2013), the National Institute for Health Research (NIHR)-funded BioResource, Clinical Research Facility and Biomedical Research Centre based at Guy's and St Thomas' NHS Foundation Trust in partnership with King's College London. CJS is funded under a grant from the Chronic Disease Research Foundation (CDRF). TDS is an NIHR Senior Investigator. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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