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Additional Information

Competing Interests

We declare that we have no competing interests relevant to this publication.

Author Contributions

Amar Joshi conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Vicent Esteve conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Adrian N Buckroyd performed the experiments, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Markus Blatter conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Frédéric HT Allain conceived and designed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Stephen Curry conceived and designed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Data Deposition

The following information was supplied regarding the deposition of related data:

Coordinates, X-ray structure factors and NMR assignments used in structure determination will be deposited with the Protein Data Bank. nPTB34 crystal structure: PDB ID - 4cko. nPTB34 NMR solution structure is in the process of being deposited.

Funding

This work was supported in part by grant support from the Biotechnological and Biological Sciences Research Council (BBSRC) to SC and from SNF-NCCR Structural Biology to FHTA. AJ is grateful for the award of a BBSRC PhD studentship. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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