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Supplemental Information

Giemsa stained cells

The arrow in the Light microscope image showing ‘transparent’ round shape bodies observed adjacent to lysed Giemsa stained cells while the nucleus and cytoplasm are stained with purple and pink colour (x200). Image restricted by condenser to maximise core illumination.

DOI: 10.7287/peerj.preprints.209v2/supp-1

DAPI Stain

Fixed cells on cover slips and stained with counterstain DAPI. Arrow point to the Spots of nuclear materials observed around the nucleus of cells in the image (x100).

DOI: 10.7287/peerj.preprints.209v2/supp-2

TEM micrographs of C3A cells infected with unknown bacteria

Bacteria are engulfed by elongated microvilli from infected epithelial cells. High magnification showing partially lysed vacuole membrane containing bacteria, indicating the ability of bacteria to escape from the endocytic vacuoles.

DOI: 10.7287/peerj.preprints.209v2/supp-3

TEM micrographs of C3A cells infected with unknown bacteria (~ 1 m m dia).

(~ 1 m m dia). Cross section of the cells monolayer showing numerous intracellular bacteria. Micrograph showing membrane ‘ruffling’ upon contact with bacteria.

DOI: 10.7287/peerj.preprints.209v2/supp-4

Additional Information

Competing Interests

The authors declare that there are no competing interests.

Author Contributions

Salwa Barkwan conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper, grant the funding to proceed in this small project.

Haroon Rasheed performed the experiments, contributed reagents/materials/analysis tools.

Oladimejii Spoloso performed the experiments, contributed reagents/materials/analysis tools.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

GenBank (EMBL)

HE994466 , HE994467

Funding

This research was supported by the BRI, Athlone Institute of Technology, Athlone, Ireland The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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