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Supplemental Information

Substitution rate between pAsa4c (JF2267) and pAsa4b (01-B522) using 1-kbp windows

A global alignment of pAsa4b and pAsa4c was performed using stretcher (available as a part of EMBOSS 6.6.0.0) ( Rice et al., 2000 ) , and a custom R script ( R Core Team, 2015 ) was used to visualize the number of substitutions by 1000-bp sliding windows ( Zeileis & Grothendieck, 2005 ) . Transparent lines represent deletions in pAsa4c compared to pAsa4b and should not be considered.

DOI: 10.7287/peerj.preprints.2067v1/supp-1

A. salmonicida subsp. salmonicida isolates used in this study

DOI: 10.7287/peerj.preprints.2067v1/supp-2

Primers used in this study

DOI: 10.7287/peerj.preprints.2067v1/supp-3

Stretcher output and Fig S1. code

DOI: 10.7287/peerj.preprints.2067v1/supp-4

Heatmap code and raw data for pAsa4b

DOI: 10.7287/peerj.preprints.2067v1/supp-5

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Katherine H Tanaka conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Antony T Vincent conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, reviewed drafts of the paper.

Mélanie V Trudel performed the experiments, reviewed drafts of the paper.

Valérie E Paquet performed the experiments, reviewed drafts of the paper.

Michel Frenette analyzed the data, wrote the paper, reviewed drafts of the paper.

Steve J Charette conceived and designed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

GenBank

pAsa4b: KT033469

pAsa4c: KT033470

Sequences will be released on June 8, 2016.

Data Deposition

The following information was supplied regarding data availability:

The raw data has been supplied as a Supplemental Dataset

Funding

This work was supported by the Fonds de la recherche québécois sur la nature et les technologies (FRQNT) (B1 scholarship to KHT and B2 scholarship to ATV), the Natural Sciences and Engineering Research Council of Canada (NSERC) (Alexander Graham Bell Canada Graduate Scholarship to KHT and Discovery grant RGPIN-2014-04595 to SJC), the Société de recherche et de développement en aquaculture continentale (SORDAC) (grant to SJC), Ressources Aquatiques Québec (RAQ) (CREATE scholarship to MVT and a grant to MF), and the Innovamer Program of the Ministère de l'Agriculture, des Pêcheries et de l'Alimentation du Québec. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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