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Supplemental Information

Supporting File S1. Timing of developmental events from individual time-lapse movies in M. scalaris

Stages and developmental events are shown in columns A, B, AC and AD. The green-headed columns and the blue-headed columns indicate developmental timing for D. melanogaster (Campos-Ortega and Hartenstein, 1997) and M. abdita (Wotton et al., 2014) respectively. Orange headed columns indicate M. scalaris data. Time-lapse IDs are listed along the top along with averages in minutes, hours and minutes (h:m). Also listed are n numbers for each event, stage duration (in minutes and in h:m) and standard deviation (STDEV) for each event. % of developmental time is show for each stage (%TED). Raw data for time-lapses 1-4 are shown in columns L-O along with details of embryo orientation, optics and time intervals used. Columns Q-T show the same time-lapses after a time adjustment has been made. In brief, 10 minutes is added for each unrecorded cleavage cycle (i.e time-lapse 4 starts at 11min in the raw data, therefore the start of the movie is C3 + 9min. By adding 9 min we arrive at the start of C3, by adding another 10 min we arrive at the start of C2, and adding another 10min the start of C1. Hence, to normalise this movie I add 9+10+10= 29min). Alternating white and grey rows mark events occurring within a stage. Stages marked in blue indicate heterochronic shifts in developmental events.

DOI: 10.7287/peerj.preprints.205v2/supp-1

Supporting Movie S1. Time-lapse movie of M. scalaris development

Time-lapse movie of a M. scalaris embryo taken using a 20x objective and DIC optics under 10S Voltalef oil. This movie corresponds to time-lapse 3 in Supporting File S1.

DOI: 10.7287/peerj.preprints.205v2/supp-2

Additional Information

Competing Interests

I have no competing interests to declare.

Author Contributions

Karl R Wotton conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.


This work was funded by the MEC-EMBL agreement for the EMBL/CRG Research Unit in Systems Biology and by grants BFU2009-10184 & BFU2012-33775 from the Spanish Ministry of Science (MICINN, now called MINECO). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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