Microsatellite loci in the tiger shark and cross-species amplification using pyrosequencing technology

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1 Laboratório de Biologia e Genética de Peixes, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil
2 Laboratório de Genética Pesqueira e Conservação, Marine Sciences Institute, Universidade Federal de São Paulo, Santos, São Paulo, Brazil
3 Lake Nona Campus, Valencia College, Orlando, Florida, United States
4 Florida Museum of Natural History, Florida Program for Shark Research, University of Florida, Gainesville, Florida, United States
5 IPMA, Portuguese Institute for the Ocean and Atmosphere, Algarve, Portugal
DOI
10.7287/peerj.preprints.2042v1
Published
Accepted
Subject Areas
Aquaculture, Fisheries and Fish Science, Conservation Biology, Genetics, Marine Biology, Zoology
Keywords
high-throughput sequencing, Galeocerdo cuvier, shark, microsatellites, population structure
Copyright
© 2016 Mendes et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Mendes NJ, Cruz VP, Ashikaga FY, Camargo SM, Oliveira C, Piercy AN, Burgess GH, Coelho R, Santos MN, Mendonça FF, Foresti F. 2016. Microsatellite loci in the tiger shark and cross-species amplification using pyrosequencing technology. PeerJ Preprints 4:e2042v1

Abstract

The tiger shark (Galeocerdo cuvier) has a global distribution in tropical and warm temperate seas, and is caught in numerous fisheries worldwide, mainly as bycatch. It is currently assessed as near threatened by the International Union for Conservation of Nature (IUCN) Red List. In this study we identified 9 microsatellite loci through next generation sequencing (454 pyrosequencing) using 29 samples from the western Atlantic. The genetic diversity of these loci was assessed and revealed a total of 48 alleles ranging from 3 to 7 alleles per locus (average of 5.3 alleles). Cross-species amplification was assessed in three other species: Carcharhinus longimanus, C. acronotus and Alopias superciliosus. Given the potential applicability of genetic markers for biological conservation, these data may contribute to the population assessment of this and other species of sharks worldwide.

Author Comment

This is a submission to PeerJ for review.