Combined proteome and transcriptome analyses reveal that Zika virus circulating in Brazil alters cell cycle and neurogenic programmes in human neurospheres

, , , , , , , , , , , , , , , , , ,
1 D'Or Institute for Research and Education (IDOR), Rio de Janeiro, Brazil
2 Institute of Biomedical Sciences, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
3 Department of Biochemistry and Tissue Biology, University of Campinas, Campinas, Brazil
4 Center for Technological Innovation, Evandro Chagas Institute, Belém, Brazil
5 Institute of Biology, Federal University of Rio de Janeiro, Rio de Janeiro, Brazil
6 Department of Biochemistry and Tissue Biology, Laboratory of Neuroproteomics, University of Campinas (UNICAMP), Campinas, SP, Brazil
7 Institute Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil
8 Federal University of Pará, Belém, Brazil
DOI
10.7287/peerj.preprints.2033v1
Published
Accepted
Subject Areas
Cell Biology, Developmental Biology, Microbiology, Molecular Biology, Neuroscience
Keywords
Zika virus, Microcephaly, Neural stem cells, Neurospheres, Proteome, Transcriptome, Br ZIKV
Copyright
© 2016 Garcez et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Garcez PP, Minardi Nascimento J, Mota de Vasconcelos J, Madeiro da Costa R, Delvecchio R, Trindade P, Correia Loiola E, M. Higa L, Cassoli J, Vitória G, Sequeira P, Sochacki J, S. Aguiar R, Thais Fuzii H, M. Bispo de Filippis A, Lídio da Silva Gonçalves Vianez Júnior J, Martins-de-Souza D, Tanuri A, Rehen SK. 2016. Combined proteome and transcriptome analyses reveal that Zika virus circulating in Brazil alters cell cycle and neurogenic programmes in human neurospheres. PeerJ Preprints 4:e2033v1

Abstract

Zika virus (ZIKV) has been associated with microcephaly and other brain abnormalities; however, the molecular and cellular consequences of Zika virus circulating in Brazil to the human brain development have not been studied yet. Here we describe alterations in human neurospheres derived from neural stem cells infected with Brazilian ZIKV. Combined proteomics and mRNA transcriptional profile analyses showed that Brazilian ZIKV, prior to induce cell death, alters cell cycle and halts neurogenic programmes, in addition to regulate transcription and protein translation due to viral replication. These results point to biological mechanisms potentially implicated in brain malformations as a result of ZIKV congenital infection.

Author Comment

This is a preprint submission to PeerJ Preprints and to a peer reviewed journal.

Supplemental Information