Raw data of bacterial counts, bioluminescence and growth in vitro and in vivo
Elbow tests of phenotypic microarray array data to determine the number of clusters appropriate for k-means clustering
Data was analysed using the DuctApe software suite.
Supplementary Fig. 2. The growth of C. rodentium ICC180 compared to its non-bioluminescent parent strain ICC169 as assessed by phenotypic microarray (PM)
Wildtype C. rodentium ICC169 (shown as purple lines) and its bioluminescent derivative ICC180 (shown as blue lines) were grown on two separate occasions using PM plates 1-20 (categorised by colour [see Key]). Differences between the growth of ICC169 and ICC180 in each individual well were analysed using the moderated t-test provided by limma . Wells in which the differences had an adjusted p-value of less than 0.5 (stringent cut-off) are shown.
Supplementary Fig. 3. Infection of larvae of the Greater Wax Moth Galleria mellonella with bioluminescent C. rodentium ICC180 can be visualised by luminometry
Groups of larvae (n = 10) of the Greater Wax Moth Galleria mellonella were infected with ~108 CFU of C. rodentium ICC169 or ICC180 and monitored for bioluminescence using a plate luminometer. Data (medians with ranges) is presented from experiments performed on 3 separate occasions and is given as relative light units [RLU] waxworm-1.