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Supplemental Information

Raw data of bacterial counts, bioluminescence and growth in vitro and in vivo

DOI: 10.7287/peerj.preprints.1993v1/supp-1

Elbow tests of phenotypic microarray array data to determine the number of clusters appropriate for k-means clustering

Data was analysed using the DuctApe software suite.

DOI: 10.7287/peerj.preprints.1993v1/supp-2

Supplementary Fig. 2. The growth of C. rodentium ICC180 compared to its non-bioluminescent parent strain ICC169 as assessed by phenotypic microarray (PM)

Wildtype C. rodentium ICC169 (shown as purple lines) and its bioluminescent derivative ICC180 (shown as blue lines) were grown on two separate occasions using PM plates 1-20 (categorised by colour [see Key]). Differences between the growth of ICC169 and ICC180 in each individual well were analysed using the moderated t-test provided by limma . Wells in which the differences had an adjusted p-value of less than 0.5 (stringent cut-off) are shown.

DOI: 10.7287/peerj.preprints.1993v1/supp-3

Supplementary Fig. 3. Infection of larvae of the Greater Wax Moth Galleria mellonella with bioluminescent C. rodentium ICC180 can be visualised by luminometry

Groups of larvae (n = 10) of the Greater Wax Moth Galleria mellonella were infected with ~108 CFU of C. rodentium ICC169 or ICC180 and monitored for bioluminescence using a plate luminometer. Data (medians with ranges) is presented from experiments performed on 3 separate occasions and is given as relative light units [RLU] waxworm-1.

DOI: 10.7287/peerj.preprints.1993v1/supp-4

Additional Information

Competing Interests

Siouxsie Wiles is an Academic Editor for PeerJ.

Author Contributions

Hannah M Read conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Grant Mills performed the experiments, reviewed drafts of the paper.

Sarah Johnson performed the experiments, reviewed drafts of the paper.

Peter Tsai analyzed the data, prepared figures and/or tables, reviewed drafts of the paper.

James Dalton performed the experiments, analyzed the data, reviewed drafts of the paper.

Lars Barquist analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Cristin G Print contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Wayne M Patrick analyzed the data, reviewed drafts of the paper.

Siouxsie Wiles conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Experiments were performed in accordance with the New Zealand Animal Welfare Act (1999) and institutional guidelines provided by the University of Auckland Animal Ethics Committee, which reviewed and approved these experiments under applications R1003 and R1496.

Funding

This work was supported by seed funding from the Maurice Wilkins Centre for Molecular Biodiscovery, and by a Sir Charles Hercus Fellowship to SW (09/099) from the Health Research Council of New Zealand. LB is supported by a Research Fellowship from the Alexander von Humboldt Stiftung/Foundation. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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