Visitors   Views   Downloads
NOT PEER-REVIEWED
"PeerJ Preprints" is a venue for early communication or feedback before peer review. Data may be preliminary.

A peer-reviewed article of this Preprint also exists.

View peer-reviewed version

Supplemental Information

Gene ontology (GO) classification of 315 salivary proteins identified in this study

The proteins were classified based on (A) subcellular localization, (B) biological processes involved and (C) molecular functions.

DOI: 10.7287/peerj.preprints.1973v1/supp-2

Gene ontology (GO) classification of top 20 up-regulated salivary proteins identified in this study

The proteins were classified based on (A) subcellular localization, (B) biological processes involved and (C) molecular functions.

DOI: 10.7287/peerj.preprints.1973v1/supp-3

Protein-protein interaction network prediction

Network (A) contains 35 proteins involved in connective tissue disorders, immunological disease and inflammatory disease in which 22 proteins were identified from the NPDR disease group. Network (B) contains 35 proteins involved in cellular movement, hematological system development and function and immune cell trafficking in which 28 proteins are identified from the PDR disease group. Network (C) contains 26 proteins involved in cellular growth and proliferation, cancer and carbohydrate metabolism in which 10 proteins are identified in PDR disease group. (ACTB = actin, cytoplasmic 1, ANXA1 = annexin A1, APOA1 = apolipoprotein A-I , CAMP = cathelicidin antimicrobial peptide, CAP1 = adenylyl cyclase-associated protein 1, CLU = clusterin, C3 = complement C3, ELANE = neutrophil elastase, ENO1 = alpha-enolase isoform 1, EZR = ezrin, GAPDH = glyceraldehyde-3-phosphate dehydrogenase isoform 2, GSN = gelsolin isoform d, HBA1/HBA2 = hemoglobin subunit alpha, HP = haptoglobin isoform 2, HSPA8 = heat shock cognate 71 kDa protein isoform 1, HSPA1A/HSPA1B = heat shock 70 kDa protein 1A/1B, LCP1 = plastin-2, , LCN1 = lipocalin-1 isoform 1, LCN2 = neutrophil gelatinase-associated lipocalin, LDHA = L-lactate dehydrogenase A chain isoform 3, LTF = lactotransferrin isoform 1, MIF = macrophage migration inhibitory factor, MMP9 = matrix metalloproteinase-9, MPO = myeloperoxidase, PKM = pyruvate kinase isozymes M1/M2 isoform c, PLTP = phospholipid transfer protein isoform a, PRDX1 = peroxiredoxin-1, PRTN3 = profilin-1, SERPINA1 = alpha-1-antitrypsin, SLPI = antileukoproteinase, S100A8 = protein S100-A8, S100A9 = protein S100-A9, TIMP1 = metalloproteinase inhibitor 1, TPM3 = tropomyosin alpha-3 chain isoform 2).

DOI: 10.7287/peerj.preprints.1973v1/supp-4

Salivary proteins that are differentially expressed in PDR disease group compared to XDR disease group

DOI: 10.7287/peerj.preprints.1973v1/supp-5

Salivary proteins that are differentially expressed in NPDR patient group compared to XDR patient group

DOI: 10.7287/peerj.preprints.1973v1/supp-6

The differentially expressed proteins identified from the study that interact with each other in the protein-protein network analysis

DOI: 10.7287/peerj.preprints.1973v1/supp-7

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Chin Soon Chee conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Khai Meng Chang conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Mun Fai Loke conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Voon Pei Angela Loo conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Visvaraja Subrayan conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Medical Ethics Committee of UMMC (Reference number: 1017.28)

Data Deposition

The following information was supplied regarding data availability:

ProteomeXchange Consortium via the PRIDE

Funding

This work is supported by University of Malaya Research Grant (UMRG) RP006C-13HTM and University of Malaya-Ministry of Education (UM-MoE) High Impact Research (HIR) Grant UM.C/625/1/HIR/MoE/CHAN/13/4 (Account No. H-50001-A000029). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


Add your feedback

Before adding feedback, consider if it can be asked as a question instead, and if so then use the Question tab. Pointing out typos is fine, but authors are encouraged to accept only substantially helpful feedback.

Some Markdown syntax is allowed: _italic_ **bold** ^superscript^ ~subscript~ %%blockquote%% [link text](link URL)
 
By posting this you agree to PeerJ's commenting policies