Elaboration of near infrared spectroscopy model for determination of water content in freeze-dried immunological veterinary medicinal products
- Published
- Accepted
- Subject Areas
- Veterinary Medicine, Statistics
- Keywords
- Karl Fischer titration, IVMPs, Near Infrared Spectroscopy
- Copyright
- © 2016 Bulenger et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2016. Elaboration of near infrared spectroscopy model for determination of water content in freeze-dried immunological veterinary medicinal products. PeerJ Preprints 4:e1858v1 https://doi.org/10.7287/peerj.preprints.1858v1
Abstract
Residual water is a critical parameter in assessing the quality of immunological veterinary medicinal products (IVMPs). In majority of the laboratories the Karl Fischer titration (KFT) is used for the determination of water content in IVMPs. However, the transfer of IVMP into titration cell without affecting the baseline drift and repeatability seems to be the main problem when using this method. In turn, Near Infrared Spectroscopy (NIRS) allows measurement of closed vials, therefore eliminating the impact of atmospheric conditions on the sample. The aim of the study was to create a calibration model based on the reference method (Karl Fischer titration) and its optimization. Five different IVMPs designated for two animal species (dogs and rabbits) were used. The model was constructed on the basis of 49 samples tested, each in triplicate (n=147). The spectra were divided in two sets: calibration and validation. Proper selection of samples and their processing allowed to obtain a model of high quality (Q-value>0.6).
Author Comment
This is an Abstract submitted to the X MKNOL Conference.