RNA interference in gene cloning/expression
Genetic Engineering and Molecular Biology Domain, Lovely Professional University, Phagwara, Punjab, India- 144411
- Published
- Accepted
- Subject Areas
- Biotechnology, Cell Biology, Molecular Biology
- Keywords
- DNA Cloning, Rnai, Protein expression, Gene expression, Protein Expression
- Copyright
- © 2016 Bhardwaj
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
- Cite this article
- 2016. RNA interference in gene cloning/expression. PeerJ Preprints 4:e1831v1 https://doi.org/10.7287/peerj.preprints.1831v1
Abstract
DNA cloning is a simple, straightforward process. It has been put into practice as a standard lab procedure for cloning the gene of interest for specific protein expression and other molecular or cellular studies. The present study is the first of its kind to hypothesize the formation of interfering recombinant plasmid during a DNA cloning reaction that is believed to interfere with the protein or gene expression. This might lead to a false conclusion in a cellular study and also may lower the protein expression during the production stage.
Author Comment
This is a preprint submission to PeerJ Preprints.