This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ PrePrints) and either DOI or URL of the article must be cited.
Cite this article
Tang Y, Luo B, Deng Z, Wang B, Liu FL, Li J, Shi W, Xie H, Li J. (2016) Mitochondrial aerobic respiration is activated during hair follicle stem cells differentiation and its dysfunction retards hair regeneration. PeerJ PrePrints4:e1769v1https://doi.org/10.7287/peerj.preprints.1769v1
Background. Emerging researches revealed the essential role of mitochondria in regulating stem/progenitor cell differentiation of neural progenitor cells, mesenchymal stem cells and other stem cells through reactive oxygen species (ROS), Notch or other signaling pathway. And inhibition of mitochondrial synthesis protein resulted in extension of hair loss upon injury. However, alteration of mitochondrial morphology and metabolic function during hair follicle stem cells (HFSCs) differentiation and how it affects hair regeneration has not been elaborated. Methods. We compared the difference between telogen bulge cells and anagen matrix cells in mitochondrial morphology and activity. Expression levels of mitochondrial ROS and superoxide dismutase 2 (SOD2) were measured for evaluating redox balance. Besides, pyruvate dehydrogenase kinase (PDK) and pyruvate dehydrogenase (PDH) were detected to present the change in energetic metabolism during differentiation. To explore the effect of the mitochondrial metabolism on regulating hair regeneration, hair growth was observed after application of a mitochondrial respiratory inhibitor upon hair plucking. Results. During HFSCs differentiation, mitochondria became elongated with more abundant organized cristae and showed higher activity in differentiated cells. SOD2 was enhanced for redox balance with relatively poised ROS expression levels in differentiated cells. PDK increased in HFSCs while differentiated cells showed enhanced PDH, indicating that respiration converted from glycolysis to oxidative phosphorylation during differentiation. Inhibiting mitochondrial respiration in differentiated hair follicle cells upon hair plucking held back hair regeneration in vivo. Conclusions. Upon HFSCs differentiation, mitochondria was elongated with more abundant cristae and showed higher activity, accompanied with activated aerobic respiration in differentiated cells for higher energy supply. And dysfunction of mitochondrial respiration delays hair regeneration upon injury.
"Following" is like subscribing to any updates related to a preprint.
These updates will appear in your home dashboard each time you visit PeerJ.
You can also choose to receive updates via daily or weekly email digests.
If you are following multiple preprints then we will send you
no more than one email per day or week based on your preferences.
Note: You are now also subscribed to the subject areas of this preprint
and will receive updates in the daily or weekly email digests if turned on.
You can add specific subject areas through your profile settings.