Identification of phenolic secondary metabolites from Schotia brachypetala Sond. (Fabaceae) and demonstration of their antioxidant activities in Caenorhabditis elegans

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1 Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Heidelberg, Germany
2 Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt
3 Department of Pharmaceutical Biology, Faculty of Pharmacy and Biotechnology, German University in Cairo, Cairo, Egypt
4 Department of Pharmacognosy, Faculty of Pharmacy, British University in Egypt, Cairo, Egypt
5 Department of Chemistry, Humboldt Universität Berlin, Berlin, Germany
6 Pharmaceutical Analytical Chemistry Department, Faculty of pharmacy, Ain Shams University, Cairo, Egypt
7 Department of Chemistry, Humboldt-Universitaet zu Berlin, Berlin, Germany
8 Department of Pharmacognosy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt
9 Pharmacy, Ain Shams University, Cairo, Egypt
10 Department of Pharmacology and Toxicology, Faculty of medicine, Umm Al-Qura University, Makkah, Saudi Arabia
DOI
10.7287/peerj.preprints.1768v1
Published
Accepted
Subject Areas
Plant Science, Pharmacology
Keywords
polyphenolics, LC/HRESI/MS/MS, Caenorhabditis elegans, antioxidant activity., Schotia brachypetala
Copyright
© 2016 Sobeh et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ Preprints) and either DOI or URL of the article must be cited.
Cite this article
Sobeh M, ElHawary EA, Peixoto H, Labib RM, Handoussa H, Swilam N, El-Khatib AH, Sharapov F, Mahmoud T, Krstin S, Linscheid MW, Singab ANB, Wink M, Ayoub N. 2016. Identification of phenolic secondary metabolites from Schotia brachypetala Sond. (Fabaceae) and demonstration of their antioxidant activities in Caenorhabditis elegans. PeerJ Preprints 4:e1768v1

Abstract

Background: Schotia brachypetala Sond. (Fabaceae) is an endemic tree of Southern Africa whose phytochemistry and pharmacology were slightly studied.The present work aimed at profiling the major phenolics compounds present in the hydro-alcoholic extract from S. brachypetala leaves (SBE) using LC/HRESI/MS/MS and NMR and prove their antioxidant capabilities using novel methods. Methods: In vitro assays; DPPH, TEAC persulfate decolorizing kinetic and FRAP assays, and in vivo assays: Caenorhabditis elegans strains maintenance, Intracellular ROS in C. elegans, Survival assay, GFP expression and Subcellular DAF-16 localization were employed to evaluate the antioxidant activity. Results: More than forty polyphenols ,including flavonoid glycosides, galloylated flavonoid glycosides, isoflavones, dihydrochalcones, procyanidins, anthocyanins, hydroxybenzoic acid derivatives, hydrolysable tannins, and traces of methylated and acetylated flavonoid derivatives were identified. Three compounds were isolated and identified from the genus Schotia for the first time, namely gallic acid, myricetin-3-O-α-L-1C4-rhamnoside and quercetin-3-O-L-1C4-rhamnoside.The tested extract was able to protect the worms against juglone induced oxidative stress and attenuate the reactive oxygen species (ROS) accumulation. SBE was also able to attenuate the levels of heat shock protein (HSP) expression. Discussion: A pronounced antioxidant activity in vivo, which can be attributed to its ability to promote the nuclear translocation of DAF-16/FOXO, the main transcription factor regulating the expression of stress response genes. The remarkable antioxidant activity in vitro and in vivo correlates to SBE rich phenolic profile.

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DOI: 10.7287/peerj.preprints.1768v1/supp-1