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Supplemental Information

Mortality of Nephrops after transportation

Post-transport mortality of adult females, 14 days after arrival in RAS following four discrete transportation events (9h immersed in a commercial vivier truck, followed by 2h cool, dark and humid emersed transport). Unbroken and broken lines denote transportation with (n=47-56) and without (n=138-144) additional cushioning respectively.

DOI: 10.7287/peerj.preprints.1747v1/supp-1

Haemolymph changes in Nephrops before and after simulated transport

Figure 2. Nephrops norvegicus. Haemolymph changes in adults shortly after removal from creels (BS; open bar), after 1h emersed (EM; gray bar) and 1h emersed with shaking (ES; dark bar). A. Serum glucose. B. Serum ammonium. C. Serum lactate. D. Total Haemocyte Count. E. Serum protein. F. Refractive Index. Different letters, or letters uncommon between bars denote significant difference between treatments at P ≤0.05% confidence limit or greater. Absence of letters, or letters common to bars denote no significant difference. Data shown + 1 SEM, n=14-24.

DOI: 10.7287/peerj.preprints.1747v1/supp-2

Haemolymph changes in Nephrops before and after simulated transport

Figure 2. Nephrops norvegicus. Haemolymph changes in adults shortly after removal from creels (BS; open bar), after 1h emersed (EM; gray bar) and 1h emersed with shaking (ES; dark bar). A. Serum glucose. B. Serum ammonium. C. Serum lactate. D. Total Haemocyte Count. E. Serum protein. F. Refractive Index. Different letters, or letters uncommon between bars denote significant difference between treatments at P ≤0.05% confidence limit or greater. Absence of letters, or letters common to bars denote no significant difference. Data shown + 1 SEM, n=14-24.

DOI: 10.7287/peerj.preprints.1747v1/supp-3

Haemolymph changes in Nephrops before and after simulated transport

Figure 2. Nephrops norvegicus. Haemolymph changes in adults shortly after removal from creels (BS; open bar), after 1h emersed (EM; gray bar) and 1h emersed with shaking (ES; dark bar). A. Serum glucose. B. Serum ammonium. C. Serum lactate. D. Total Haemocyte Count. E. Serum protein. F. Refractive Index. Different letters, or letters uncommon between bars denote significant difference between treatments at P ≤0.05% confidence limit or greater. Absence of letters, or letters common to bars denote no significant difference. Data shown + 1 SEM, n=14-24.

DOI: 10.7287/peerj.preprints.1747v1/supp-4

Additional Information

Competing Interests

Magnus L Johnson is an Academic Editor for PeerJ.

Author Contributions

Adam Powell conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables.

Daniel M Cowing conceived and designed the experiments, performed the experiments, analyzed the data, reviewed drafts of the paper.

Susanne P Eriksson contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Magnus L Johnson performed the experiments, reviewed drafts of the paper.

Data Deposition

The following information was supplied regarding data availability:

Please see supplemental files

Funding

These data were generated as part of an ASSEMBLE grant “NephRef” awarded to the Hull University and Swansea University authors based at the The Sven Lovén Centre for Marine Sciences Kristineberg, and from the “NEPHROPS” project (EU Framework 7, Research for the benefit of SME-AG, number 286903). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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