In  vitro evaluation of antimicrobial activity of 1-lauroyl-rac-glycerol on Candidaalbicans biofilms

Emily Chen; Dalia Seleem; Bruna Benso; Vanessa Pardi; Ramiro M Murata

doi:10.7287/peerj.preprints.1717v1

In vitro evaluation of antimicrobial activity of 1-lauroyl-rac-glycerol on Candidaalbicans biofilms

Emily Chen^1,2, Dalia Seleem², Bruna Benso³, Vanessa Pardi^1,2,4, Ramiro M Murata ²

1 Herman Ostrow School of Dentistry, University of Southern California, Los Angeles, California, United States

2 Department of Periodontology Diagnostic Sciences, Dental Hygiene and Biomedical Science, University of Southern California, Los Angeles, CA, United States

3 Department of Physiological Sciences, Piracicaba Dental School, State University of Campinas, Piracicaba, SP, Brazil

4 Herman Ostrow School of Dentistry, University of Southern California, Los Angeles, Ca, USA

DOI: 10.7287/peerj.preprints.1717v1

Published: 2016-02-06
Accepted: 2016-02-06

Subject Areas: Cell Biology, Microbiology, Dentistry
Keywords: Candida albicans, in vitro, antimicrobial agent, monolaurin, biofilms., virulence factors, proteolytic enzymes, oral candidiasis, MIC/MFC, host inflammatory response

Copyright: © 2016 Chen et al.
Licence: This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ PrePrints) and either DOI or URL of the article must be cited.

Cite this article: Chen E, Seleem D, Benso B, Pardi V, Murata RM. 2016. In vitro evaluation of antimicrobial activity of 1-lauroyl-rac-glycerol on Candidaalbicans biofilms . PeerJ PrePrints 4:e1717v1 https://doi.org/10.7287/peerj.preprints.1717v1

Abstract

Monolaurin (also known as glycerol monolaurate) is a natural compound found in coconut oil and is known for its protective biological activities as an antimicrobial agent. The nature of oral candidiasis and the increased antifungal resistance, culminate the need for investigating novel antifungal therapeutic agents. In this study, we examine the antifungal activity of monolaurin against Candida albicans biofilms (strain ATCC: SC5314/MYA2876) in vitro and how monolaurin may alter specific host inflammatory markers, such as gene expression of inflammatory cytokines IL-1α and IL-1β, as illustrated in co-culture models. The results from three groups were compared: 1- monolaurin (in the range of 3.9-2500 μM), positive control fluconazole (322 μM), and vehicle control group 1% Ethanol (v/v) The MIC and MFC of monolaurin were in the range 62.5-125 µM and 125-250 µM, respectively. The results show significant reduction in Log (CFU/ ml) of biofilms treated with 1250 and 2500 µM of 1- monolaurin when compared to the control groups. There was also a significant down-regulation of IL-1α and IL-1β in the biofilms treated with monolaurin. It can be concluded that monolaurin has a potential antifungal activity against C. albicans and can modulate the host’s pro-inflammatory response.