The genome and transcriptome of the Phalaenopsis yield insights into floral organ development and flowering regulation
- Published
- Accepted
- Subject Areas
- Genomics, Plant Science
- Keywords
- Phalaenopsis, draft genome, PhAGL6b, flower organ development, flowering time
- Copyright
- © 2016 Huang et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ PrePrints) and either DOI or URL of the article must be cited.
- Cite this article
- (2016) The genome and transcriptome of the Phalaenopsis yield insights into floral organ development and flowering regulation. PeerJ PrePrints 4:e1707v1 https://doi.org/10.7287/peerj.preprints.1707v1
Abstract
Phalaenopsis orchid is an important potted flower with high economic value around the world. We report the 3.1 Gb draft genome assembly of an important winter flowering Phalaenopsis ‘KHM190’ cultivar. We generated 89.5 Gb RNA-seq and 113 million sRNA-seq reads to use these data to identify 41,153 protein-coding genes and 188 miRNA families. We also generated a draft genome for Phalaenopsis pulcherrima ‘B8802’, a summer flowering species, via resequencing. Comparison of genome data between the two Phalaenopsis cultivars allowed the identification of 691,532 single-nucleotide polymorphisms. In this study, we reveal the key role of PhAGL6b in the regulation of flower organ development involves alternative splicing. We also show gibberellin pathways that regulate the expression of genes control flowering time during the stage in reproductive phase change induced by cool temperature. Our work should contribute a valuable resource for the flowering control, flower architecture development, and breeding of the Phalaenopsis orchids.
Author Comment
This is a submission to PeerJ for review.