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Supplemental Information

Appendix 3 - Detailed methods for the literature review

DOI: 10.7287/peerj.preprints.1688v1/supp-1

Figure S1. Photography showing the experimental setup

Photography showing the experimental setup. The 2 L glass bottles with the bacterial communities are randomly distributed among the two water basins and floating within the closed PVC cylinders. Each basin contains about 1000 L of water, mimicking temperature conditions in a shallow lake.

DOI: 10.7287/peerj.preprints.1688v1/supp-2

Table S1: Summery of studies included in the literature review

DOI: 10.7287/peerj.preprints.1688v1/supp-3

Figure S2. The temperature in the water basins over the course of the experiment in degree Celsius

The temperature in the water basins over the course of the experiment in degree Celsius. The temperature was logged continuously with a HOBO® temperature logger, submerged in a glass bottle that is identical to the experimental units. The dashed line at the beginning of the graph shows the period before the bottles were transferred to the water bathes. The vertical grey dashed lines demark the sampling dates. The three experimental periods are color coded: blue is the regrowth phase before the first sampling, red is the period between the first and the second sampling and red is the period after the second sampling to the end of the experiment. The horizontal straight lines show the respective average temperatures.

DOI: 10.7287/peerj.preprints.1688v1/supp-4

Figure S3. Sensitivity of the calculated diversity to rarefaction level

Figure_S_1.pdf Sensitivity of the calculated diversity to rarefaction level. The graph shows pairwise correlations between the effective number of species calculated on the unrarefied dataset and at 4 rarefaction levels (10 000, 7000, 4000 and 1000 reads). The lower triangle shows the pairwise scatterplots, the upper panels give the corresponding r-squares.

DOI: 10.7287/peerj.preprints.1688v1/supp-5

Figure_S_1.pdf Figure S4. Bacterial cell abundance (cells*ml-1) over the course of the experiment for all lakes and dilutions

Bacterial cell abundance (cells*ml-1) over the course of the experiment for all lakes and dilutions. Each panel corresponds to a dilution treatment labeled as in main Figure 1. The grey dashed vertical lines demark the sampling dates.

DOI: 10.7287/peerj.preprints.1688v1/supp-6

Figure_S_1.pdf Figure S5. Pairwise correlations of the three diversity metrics, by lake

Figure_S_1.pdf Pairwise correlations of the three diversity metrics, by lake. The lower triangle shows the pairwise scatterplots, the upper triangle shows the Pearson correlation coefficients by lake. The diversity values are the averages over the three sampling dates.

DOI: 10.7287/peerj.preprints.1688v1/supp-7

Figure_S_1.pdf Figure S6. Cell abundance, stability of cell abundance, DIN concentration and multifunctionality as functions of the three dimensions of diversity

Figure_S_1.pdf Cell abundance, stability of cell abundance, DIN concentration and multifunctionality as functions of the three dimensions of diversity. Diversity is averaged over the three sampling dates. Spearman rank correlation coefficients and p-value are given in each panel. effN = effective number of species, PD = phylogenetic diversity, FuncDiv = functional diversity.

DOI: 10.7287/peerj.preprints.1688v1/supp-8

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Fabian Roger conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Stefan Bertilsson conceived and designed the experiments, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Silke Langenheder conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Omneya Ahmed performed the experiments, analyzed the data, wrote the paper, reviewed drafts of the paper.

Lars Gamfeldt conceived and designed the experiments, contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The DNA sequences have not been submitted to a sequence repository yet.

Data Deposition

The following information was supplied regarding data availability:

The full code, including all the raw data except the sequencing data, is provided in Appendix 2, which can be accessed at https://github.com/FabianRoger/Roger_et_al_Supplementary.

Funding

The authors received no funding for this work.


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