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Supplemental Information

Major histocompatibility complex paralogs

Supplementary Figure 1. Multiple DNA sequence alignment of 16 putative MHC II DRB alleles from the reference little brown bat genome, indicating the location of primers and the boundaries of exon 2. The red box indicates where the two forward primers differ.

DOI: 10.7287/peerj.preprints.1662v1/supp-1

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Jonathan M. Palmer conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables.

Leah K. Berkman conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables.

Paula E Marquardt contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Deahn M. Donner contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Michelle A. Jusino performed the experiments, reviewed drafts of the paper.

Daniel L. Lindner contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Samples were collected in a humane and ethical manner following accepted conventions at the time of collection (i.e., 2013). Specifically, sampling protocols outlined by the American Society of Mammologists (Sikes et al. 2011). Trained and experienced USDA forest service (USFS) employees handled and collected samples on USFS lands as part of a larger habitat monitoring program, and we acquired samples under this program for the study. The USFS did not have an established institutional animal care and use committee at the time of sampling. Because samples were collected by federal employees on federal lands under another project, the SUNY College of Environmental Science and Forestry’s Institutional Animal Care and Use Committee review board issued a waiver from an animal care and use protocol.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

Sequence data was deposited in the NCBI SRA: accession number SRP067348

Data Deposition

The following information was supplied regarding data availability:

Scripts to process the data are publicly available thru GitHub: https://github.com/nextgenusfs/mhc_cluster

Funding

The project was supported by a grant from the Northeastern States Research Cooperative and funding from the USDA Forest Service. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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