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Supplemental Information

Severe hypoxia causes a reduction in U87 cell viability

U87 cells were exposed to 0.1% and 20% O2 for the indicated time points. Cell survival was assessed by Annexin/PI staining and flow cytometry.

DOI: 10.7287/peerj.preprints.1652v1/supp-2

Severe hypoxia causes a transient G1 phase arrest in D566 cells

Representative histograms of D566 cell cycle following exposure to 0.1% and 20% O2 for the indicated time points. Cell cycle distribution was analysed using flow cytometry.

DOI: 10.7287/peerj.preprints.1652v1/supp-3

Cell cycle arrest can be induced in GBM cells

Cells were incubated in 20% O2 and exposed to 10µM etoposide for 24 h. Cell cycle distribution was analysed using flow cytometry.

DOI: 10.7287/peerj.preprints.1652v1/supp-4

Additional Information

Competing Interests

Violaine See is an Academic Editor for PeerJ.

Author Contributions

Rosalie Richards conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Michael D Jenkinson contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Brian J Haylock contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Violaine See conceived and designed the experiments, wrote the paper, reviewed drafts of the paper.

Human Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The Walton Research Tissue Bank, Walton Centre NHS Foundation Trust. National Research Ethics Service (11/WNo03/2).

Data Deposition

The following information was supplied regarding data availability:

figshare; https://figshare.com/s/6bfd585c89dd5c321f03

Funding

The work was funded by Naseem's Manx Brain Tumour Charity. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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