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Supplemental Information

Raw data for Figure 1B

This file includes three sheets: "Original data" sheet has rhodamine fluorescence intensity measurements for all examined oocytes. Net rhodamine fluorescence intensity is equal to cytoplasmic intensity subtracted by background intensity (outside the oocyte); "Statistics" sheet has all the data for statistics and the P value from T test. "Graph" sheet has AVEs and SEMs for the graph corresponding to Figure 1B.

DOI: 10.7287/peerj.preprints.1566v1/supp-1

Raw data corresponding to Figure 3B

This file includes three sheets: "Original data" sheet has numbers and percentages of oocytes at each stage (GV, GV-like, Pre-MI, MI, AI and TI) at 8 hour of IVM from three repeats; "Statistics" sheet has all the GV-like or MI percentages for statistics and the P value from T test; "Graph" sheet has the AVEs and SEMs for the graph corresponding to Figure 3B.

DOI: 10.7287/peerj.preprints.1566v1/supp-2

Raw data corresponding to Figure 3D

This file includes three sheets: "Original data" sheet has all the measurements of MI spindle length or width (in pixel) at 8 hour of IVM from three repeats; "Statistics" sheet has all the measurements of MI spindle length (in pixel) for statistics and the P value from T test. Statistics for spindle width is not shown since it's not significant; "Graph" sheet has all the AVEs and SEMs (in pixel or μm) for the graph corresponding to Figure 3D.

DOI: 10.7287/peerj.preprints.1566v1/supp-3

Raw data corresponding to Figure 3F

This file includes three sheets: "Original data" sheet has all the numbers and percentages of oocytes at each stage (GV, GV-like, Pre-MI, MI, AI and TI) at 8 hour of IVM from three repeats; "Statistics" sheet has all the Pre-MII or total MII (MII) percentages for statistics and the P value from T test; "Graph" sheet has all the AVEs and SEMs for the graph corresponding to Figure 3F.

DOI: 10.7287/peerj.preprints.1566v1/supp-4

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Ruichao Li performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Zhen Jin performed the experiments, reviewed drafts of the paper.

Leilei Gao performed the experiments, reviewed drafts of the paper.

Peng Liu performed the experiments, reviewed drafts of the paper.

Zhixia Yang conceived and designed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Dong Zhang conceived and designed the experiments, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Animal Care and Use Committee of Nanjing Medical University (approval No:14030158)

Data Deposition

The following information was supplied regarding data availability:

Raw datasets have been uploaded as supplemental files

Funding

This research is supported by the National Key Basic Research Program of China ("973" program, from Ministry of Science and Technology of China, grant No: 2013CB945504), the General Program of National Natural Science Foundation of China (from National Natural Science Foundation Committe of China, grant No: 31271441 and 31471406). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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