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Supplemental Information

Microsoft Word - Sales_2011_rev.doc Figure 1: Sibutramine inhibition of acetic acid-induced abdominal writhes in mice

Microsoft Word - Sales_2011_rev.doc Separate groups of 6 mice were administered vehicle or a dose of drug p.o. followed 55 min later by an i.p. injection of 0.55% acetic acid. The total number of writhes made by each mouse was counted between 5 and 20 min after acetic acid administration. Data are expressed as the mean number of writhes during the 15-min observation period. A group was treated with aspirin (ASA) 200 mg/kg and 4 other groups were treated with increasing doses of sibutramine hydrochloride monohydrate (0.1, 0.5, 1.5, and 5.0 mg/kg). Statistical significant difference from control is depicted when present (One-way ANOVA with Tukey as post-hoc test). *** p < 0.001

DOI: 10.7287/peerj.preprints.1544v2/supp-1

Microsoft Word - Sales_2011_rev.doc Figure 2: Sibutramine reversal of carrageenan-induced acute thermal hyperalgesia

Microsoft Word - Sales_2011_rev.doc Plantar test was performed as described in methods. SHAM group (SHAM) had no pharmacological treatment. Control group had a 1.0 mg carrageenan plantar injection in right hindpaw 2 h before the test. Treatment groups (SIB 0.1, SIB 0.5, SIB 1.5, SIB 5.0, AMI 10, and INDO 10) were treated with sibutramine 0.1 to 5.0 mg/kg (B), amitriptyline 10 mg/kg or indomethacin 10 mg/kg (A) at the same time of carrageenan injection. A group had sibutramine 1.5 mg/kg treatment but no inflammatory stimulus (SHAM SIB). Reaction time to thermal stimulus (mean ± SD) was plotted. Statistical significant difference from control is depicted when present (One-way ANOVA with Tukey as post- hoc test). * p < 0.05; ** p < 0.01; *** p < 0.001

DOI: 10.7287/peerj.preprints.1544v2/supp-2

Microsoft Word - Sales_2011_rev.doc Figure 3: Neither reserpine nor yohimbine did inhibit sibutramine effect

Microsoft Word - Sales_2011_rev.doc Plantar test was performed as described in methods. Animals were pre-treated with reserpine (Sigma), 5.0 mg/kg, administered i.p. (0.2 ml/animal), 6 h before plantar test (B), or with yohimbine 2.0 mg/kg at the same time of p.o. drug treatment (A). Treatment groups (SIB 1.5) were treated with sibutramine 1.5 mg/kg at the same time of carrageenan injection. Reaction time to thermal stimulus (mean ± SD) was plotted. Statistical significant difference from control is depicted when present (One-way ANOVA with Tukey as post- hoc test). * p < 0.05; ** p < 0.01; *** p < 0.001

DOI: 10.7287/peerj.preprints.1544v2/supp-3

Microsoft Word - Sales_2011_rev.doc Yohimbine did not inhibit sibutramine effect (raw dataset)

Microsoft Word - Sales_2011_rev.doc Plantar test was performed as described in methods. Animals were pre-treated with yohimbine 2.0 mg/kg at the same time of p.o. drug treatment (io). Treatment groups (sib) were treated with sibutramine 1.5 mg/kg at the same time of carrageenan injection. Reaction time to thermal stimulus in seconds.

DOI: 10.7287/peerj.preprints.1544v2/supp-4

Reserpine did not inhibit sibutramine effect (raw dataset)

Microsoft Word - Sales_2011_rev.doc Microsoft Word - Sales_2011_rev.doc Plantar test was performed as described in methods. Animals were pre-treated with reserpine (Sigma), 5.0 mg/kg, administered i.p. (0.2 ml/animal), 6 h before plantar test (RES). Treatment groups (SIB) were treated with sibutramine 1.5 mg/kg at the same time of carrageenan injection. Reaction time to thermal stimulus in seconds.

DOI: 10.7287/peerj.preprints.1544v2/supp-5

Microsoft Word - Sales_2011_rev.doc Sibutramine inhibition of acetic acid-induced abdominal writhes in mice (raw dataset)

Microsoft Word - Sales_2011_rev.doc Separate groups of 6 mice were administered vehicle or a dose of drug p.o. followed 55 min later by an i.p. injection of 0.55% acetic acid. The total number of writhes made by each mouse was counted between 5 and 20 min after acetic acid administration. Data are expressed as the number of writhes during the 15-min observation period. A group was treated with aspirin (ASA) 200 mg/kg and 4 other groups were treated with increasing doses of sibutramine hydrochloride monohydrate (0.1, 0.5, 1.5, and 5.0 mg/kg).

DOI: 10.7287/peerj.preprints.1544v2/supp-6

Microsoft Word - Sales_2011_rev.doc Sibutramine reversal of carrageenan-induced acute thermal hyperalgesia (raw dataset)

Microsoft Word - Sales_2011_rev.doc Plantar test was performed as described in methods. SHAM group (SHAM) had no pharmacological treatment. Control group had a 1.0 mg carrageenan plantar injection in right hindpaw 2 h before the test. Treatment groups (SIB 0.1, SIB 0.5, SIB 1.5, SIB 5.0, AMI 10, and INDO 10) were treated with sibutramine 0.1 to 5.0 mg/kg, amitriptyline 10 mg/kg or indomethacin 10 mg/kg at the same time of carrageenan injection. A group had sibutramine 1.5 mg/kg treatment but no inflammatory stimulus (SHAM SIB). Reaction time to thermal stimulus in seconds.

DOI: 10.7287/peerj.preprints.1544v2/supp-7

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Maria Sales performed the experiments, wrote the paper.

Karolinne S Figueiredo performed the experiments, wrote the paper.

Juvenia B Fontenele conceived and designed the experiments, analyzed the data, wrote the paper, reviewed drafts of the paper, consulting.

Glauce SB Viana contributed reagents/materials/analysis tools, wrote the paper.

Francisco HC Felix conceived and designed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

A copy of the approval has been uploaded as a supp file.

Data Deposition

The following information was supplied regarding data availability:

Raw data published as supplemental files.

Funding

The authors received no funding for this work.


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