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Supplemental Information

Supplementary data for figure 1A, 1B, 1E and 3.xlsx

Tables of raw data for Figure 1: MSCs characterization; A) Immunophenotyping of BM-MSC from young and aged donors (n=3). Representative graphs were all positive for CD105, CD90, CD44 and negative for CD19; B) Cumulative population doubling (CPD) of MSC expanded under normal and hypoxic conditions at p15; E) Representative reverse-transcription polymerase chain reaction run on 2% of agarose gel showing expression of aP2 and adiponectin by adipocytes and RUNX2 and osteopontin by osteocytes cells, induced from human bone-marrow mesenchymal stem cells. F) Senescence-associated β-Gal activity in MSC of normal vs Hypoxic at p15. Figure 4: Reproducibility of NGS and comparison of miRNA expression between NGS and qPCR analysis.

DOI: 10.7287/peerj.preprints.1526v1/supp-1

Additional Information

Competing Interests

Soon Keng Cheong is currently an employee of Persiaran Cyberpoint Selatan, Cryocord Sdn Bhd. However, his position in CryoCord does not influence the design and output of the study. The rest of the authors declare that they have no competing interests.

Author Contributions

Norlaily Mohd Ali conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Lily Boo performed the experiments, analyzed the data, reviewed drafts of the paper.

Swee Keong Yeap conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Huynh Ky performed the experiments.

Dilan A Satharasinghe analyzed the data.

Woan Charn Liew performed the experiments.

Han Kiat Ong analyzed the data, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Soon Keng Cheong contributed reagents/materials/analysis tools.

Tunku Kamarul contributed reagents/materials/analysis tools.

Data Deposition

The following information was supplied regarding data availability:

database (GEO accession number: GSE67630).


The project was supported by the University of Malaya’s HIR-MOHE research grant initiative UM.C/625/1/HIR/MOHE/CHAN-03. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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