Supplementary data for figure 1A, 1B, 1E and 3.xlsx
Tables of raw data for Figure 1: MSCs characterization; A) Immunophenotyping of BM-MSC from young and aged donors (n=3). Representative graphs were all positive for CD105, CD90, CD44 and negative for CD19; B) Cumulative population doubling (CPD) of MSC expanded under normal and hypoxic conditions at p15; E) Representative reverse-transcription polymerase chain reaction run on 2% of agarose gel showing expression of aP2 and adiponectin by adipocytes and RUNX2 and osteopontin by osteocytes cells, induced from human bone-marrow mesenchymal stem cells. F) Senescence-associated β-Gal activity in MSC of normal vs Hypoxic at p15. Figure 4: Reproducibility of NGS and comparison of miRNA expression between NGS and qPCR analysis.