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Truelove N, Behringer D, Butler IV M, Preziosi RF.2015. Characterization of two microsatellite PCR multiplexes for high throughput genotyping of the Caribbean spiny lobster, Panulirus argus. PeerJ PrePrints3:e1498v2https://doi.org/10.7287/peerj.preprints.1498v2
The spiny lobster Panulirus argus supports one of the most economically important commercial fisheries in the Caribbean, yet its sustainable management is problematic due to uncertainty regarding levels of population connectivity among Caribbean nations. We developed two microsatellite multiplex panels for P. argus to assist in future conservation genetics research studies of this important Caribbean species. Significant deviations from Hardy–Weinberg equilibrium were observed at locus Par7 in multiplex 1 and loci Fwc08 and Fwc17 in multiplex 2. No evidence of linkage disequilibrium was observed. All 12 loci were used in both microsatellite multiplexes were polymorphic, with an average of 12 alleles per locus (ranging from 3 to 29 alleles per locus) and HO ranging from 0.368 to 0.921. These two microsatellite multiplexes will be a valuable resource for ongoing and future studies of conservation genetics in the Caribbean spiny lobster, Panulirus argus.
Table 1 has been added as a supplemental file.
Table 1: Characteristics of two microsatellite multiplexes for the Caribbean spiny lobster Panulirus argus
Number of alleles (NA), range of allele sizes, observed (HO) and expected (HE) heterozygosity, Hardy–Weinberg Equilibrium P-values (PHWE) are based on 56 individuals. The types of fluorescent labels used on forward primers are indicated (6-FAM, NED, PET, VIC). Multiplexes, fluorescent labels, and significant deviations from HWE after using the Bonferroni correction for multiple comparisons are indicated in bold.